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OAS accession Detail for 0278773
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| accessions_id: | 0278773 | archive |
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| Title: | Biogeochemistry and microbiology from the R/V Hermano Gines cruises in the Cariaco Basin from 1995 to 2015 (CARIACO Ocean Time-Series Program) (NCEI Accession 0278773) |
| Abstract: | This dataset contains chemical and physical data collected on B/O Hermano Gines during cruise HG93_CARIACO in the Caribbean Sea from 1995-11-14 to 2015-11-15. These data include Ammonium, Fe, Mn, Nitrate, Nitrite, Sulfur, depth, dissolved Oxygen, methane, and reactive phosphorus (PO4). The instruments used to collect these data include Gas Chromatograph, Niskin bottle, and Spectrophotometer. These data were collected by Jaimie Rojas, Ramon Varela, and Yrene Astor of Estacion de Investigaciones Marinas de Margarita, Dr Gordon T. Taylor and Dr Mary I. Scranton of Stony Brook University - SoMAS, and Dr Frank Muller-Karger, Dr Kent Fanning, and Kristen N. Buck of University of South Florida as part of the "CARIACO Ocean Time-Series Program (CARIACO)" project and "Ocean Carbon and Biogeochemistry (OCB)", "Ocean Time-series Sites (Ocean Time-series)", and "U.S. Joint Global Ocean Flux Study (U.S. JGOFS)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-06-07. The following is the text of the dataset description provided by BCO-DMO: Cariaco basin biogeochemistry and microbiology Dataset Description: Biogeochemistry and microbiology measurements in the Cariaco Basin. Microbiology sampling is conducted during special CARIACO cruises (distinct from the monthly, core sampling, time-series cruises). The specialized microbiology cruises are usually during May and November, and can be opportunistic or process-driven. There are typically at least 2 cruises per year, during which different variables are sampled and at different depths from the standard monthly CARIACO cruises. Bacteria production data are also reported for the microbiology cruises. Methodology published at CARIACO site (http://imars.usf.edu/publications/methods-cariaco) CARIACO Field Program general description (http://www.imars.usf.edu/cariaco) |
| Date received: | 20190607 |
| Start date: | 19951114 |
| End date: | 20151115 |
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| West boundary: | -65.587 |
| East boundary: | -64.54 |
| North boundary: | 10.716 |
| South boundary: | 10.45 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Sampling Ocean time series samples are collected in standard 8L Niskin bottles. For samples in and below the oxycline, a nitrogen line is attached to the upper air vent to prevent air from entering the bottle during subsampling. Samples for live analysis are first transferred without headspace to a 1L glass sample bottle with Teflon standard taper stopper. In the ship's lab, subsamples are transferred to 25 or 40 ml incubation vials, also under nitrogen. All vials are filled from the bottom with overflow of about 3 vial volumes and then sealed with no headspace. Low molecular weight fatty acids: Volatile fatty acids are measured using the technique developed by Yang (1991), Yang et al. (1993) and Wu and Scranton (1994). Detection limits are about 1 (M for acetate). However, in some cases, deep water values are lower than 1 micromolar for acetate in which case we have estimated blanks in individual files. Samples are poisoned with 1 ml 10N KOH per liter. Fatty acid uptake rate constants: Acetate uptake rate constants are determined using radiolabeled tracers as described by Wu and Scranton (1994). Incubations are done anoxically in the dark in screw-top septum vials. Uptake includes both conversion of isotope to CO2 (respiration) and to biomass which can be filtered onto a 0.2 m Nuclepore filter (incorporation). CH4: CH4 is assayed by gas chromatography using the vial equilibration technique of Johnson et al. (1990) and a Carle 211AC gas chromatograph. Samples are poisoned by addition of 10N KOH solution at a rate of 200 l per 50 ml vial. H2S: Samples for sulfide analysis are taken in well-flushed glass syringes without bubbles and are injected into vials containing Zn-acetate. Upon return to the laboratory, the ZnS is dissolved and is analyzed spectrophotometrically by the method of Cline (1969). Microbial census: Abundances of remineralizers (bacteria) and regenerators (protozoa) are determined using microscopic censuses. Preserved samples (2% formaldehyde) are stained with a fluorochrome (DAPI or acridine orange) and captured on the appropriate porosity Nuclepore membrane (0.2 or 0.8 m). Filter-retained cells are enumerated and sized by epifluorescence microscopy according to Taylor et al. (1986). Larger, less abundant protozoa are enumerated on settled samples using inverted microscopy. Abundance and distribution of methanogens are determined by an autofluorescence microscopic technique whereby the fluorescence of coenzymes F420 and F350 in cells produced by two sets of excitation and barrier filters is considered presumptive identification of methanogens (Doddema and Vogels 1978). Bacterial production: Bacterial incorporation is measured using 3H-leucine incorporation as described by Kirchman (1993). Triplicate samples are incubated for 10-12 h in gas-tight screw-top vials to minimized alteration of the redox potential. Time course experiments have confirmed that uptake is linear for at least 15 hours. Due to the fact that some important anaerobic bacteria appear to not take up exogenous thymidine under anoxic conditions (McDonough et al. 1986; Gilmour et al. 1990), the more common method of Fuhrman and Azam (1982) is inappropriate for this system. |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2023-05-26 04:40:06+00 |
| Editdate: | 2023-05-26 04:40:44+00 |