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OAS accession Detail for 0278691
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Title: Bacterial cell counts and Dissolved Organic Carbon (DOC) measurements from R/V Atlantis AT32, AT34, AT38, and AT39-06 in the western North Atlantic Ocean (35°N to 57°N; 45°W) in Nov. 2015, May 2016, Sep 2017, Mar/Apr 2018 (NCEI Accession 0278691)
Abstract: This dataset contains biological, chemical, and physical data collected on R/V Atlantis during cruises AT34, AT38, and AT39-06 from 2016-05-11 to 2018-03-20. These data include Carbon, bacterial abundance, depth, and dissolved organic Carbon. The instruments used to collect these data include Fluorescence Microscope, Niskin bottle, Shimadzu TOC-V Analyzer, and Shipboard Incubator. These data were collected by Craig Carlson of University of California-Santa Barbara as part of the "Tracking the temporal and spatial variability of dissolved organic matter, its diagenetic state and bioavailability during various bloom states in the North Atlantic (DOM_SeasonalDynamics)" project and "North Atlantic Aerosols and Marine Ecosystems Study (NAAMES)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-09-22.

The following is the text of the dataset description provided by BCO-DMO:

bacterial abundance and DOC

Dataset Description:
This dataset includes analyses from Niskin bottle samples collected on R/V Atlantis cruises AT32, AT34, AT38 and AT39-6 as part of the NASA NAAMES campaign (2015-2018). Reported are bacterial abundances and Dissolved Organic Carbon (DOC) measurements pre- and post-experiment. Remineralization experiments were used to examine the ability of natural assemblages of bacteria to utilize in situ DOM as well as different algal-derived substrates.
Date received: 20200922
Start date: 20160511
End date: 20180320
Seanames:
West boundary: -46.148
East boundary: -37.514
North boundary: 56.341
South boundary: 39.187
Observation types: biological, chemical, physical
Instrument types: fluorescence microscope, Niskin bottle
Datatypes: BACTERIA - BACTERIAL DENSITY, CARBON, DEPTH - OBSERVATION, DISSOLVED ORGANIC CARBON
Submitter:
Submitting institution: Biological and Chemical Oceanography Data Management Office
Collecting institutions: University of California - Santa Barbara
Contributing projects:
Platforms: Atlantis (33AT)
Number of observations:
Supplementary information: Acquisition Description:
Bioassays were conducted at each station using water collected within 10 m depth horizon. Water was gently gravity-filtered serially through 142 mm PC filtration cartridges loaded with either a 1.2- or a 0.2-µm mixed cellulose ester membrane filter. The 1.2 µm filtrate was retained as a bacterioplankton inoculum with diluted large phytoplankton and nanoflagellate grazers while the 0.2 µm fraction was retained as particle-free DOM substrate. When possible, the filtration rig was directly attached to Niskin bottles with platinum-cured silicon tubing and the filtrate was collected into PC carboys. Otherwise, unfiltered water from the Niskin was first drawn into a PC carboy and then filtered into another PC carboy. Each bioassay was initiated by combining the 1.2 µm filtrate with the 0.2 µm filtrate in a 30:70 ratio. A pair of incubation bottles were then rinsed with this water and subsequently filled. Ammendments were added if applicable. 8 pre-combusted (4 hours at 450°C) 40 mL EPA borosilicate glass incubation vials were also rinsed and filled with initial incubation water. Bioassays were maintained in Fisherbrand Isotemp BOD refrigerated incubators. They were kept in darkness for up to 81 days at temperatures as near in situ as logistically possible (±1.5°C). Incubation bottles were sampled over the duration of each campaign, while vials were sacrificially sampled for organic carbon during and beyond the duration of each campaign. Bacterioplankton carbon (BC, µmol C / L) refers to the carbon content of a population at any given time. BC at the initial and stationary growth conditions of each bioassay were estimated by applying empirical carbon conversion factors (CCFs, fg C / cell) to POC estimates. The dissolved organic carbon (DOC) fraction in a bioassay, was estimated by subtracting bacterioplankton cell carbon (µmol C / L) from TOC measurements.
Availability date:
Metadata version: 1
Keydate: 2023-05-24 05:02:23+00
Editdate: 2023-05-24 05:03:09+00