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OAS accession Detail for 0278503
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| accessions_id: | 0278503 | archive |
|---|---|
| Title: | Biological, physical, and chemical data from surface Transect 5 on MV1405 (IRN-BRU) from 2014-07-13 to 2014-07-14 (NCEI Accession 0278503) |
| Abstract: | This dataset contains biological, chemical, meteorological, optical, and physical data collected on R/V Melville during cruise MV1405 from 2014-07-13 to 2014-07-14. These data include Iron, Manganese, PAR, Silicate, barometric pressure, depth, fluorescence, nitrate plus nitrite, reactive phosphorus (PO4), sea surface temperature, and wind_speed. The instruments used to collect these data include Anemometer, Automated DNA Sequencer, Flow Injection Analyzer, Inductively Coupled Plasma Mass Spectrometer, PCR Thermal Cycler, Sea-Bird SBE 45 MicroTSG Thermosalinograph, and Turner Designs Fluorometer 10-AU. These data were collected by Tyler Coale of J. Craig Venter Institute, Phoebe Dreux Chappell of Old Dominion University, and Claire P. Till and Kenneth W. Bruland of University of California-Santa Cruz as part of the "Accomplishment Based Renewal: An iron limitation mosaic within the central California Current System (iron limitation mosaic)" and "OCE-RIG: Developing Molecular Bioassays for Evaluating Iron Status of Environmentally Relevant Diatoms (DiatomFeMolBio)" projects. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-07-06. The following is the text of the dataset description provided by BCO-DMO: Dataset Description: The complete dataset for the ships underway system includes the following parameters: BOTTOM_DEPTH, TRUE_WIND_SPEED, BAROM_PRES, PAR, CORR_SST, CORR_SALINITY, FLUORESCENCE. This dataset is available via the Rolling Deck to Repository: rvdata.us/search/cruise/MV1405 Diatom 18S Amplicon Sequencing from California Current System Mesoscale Eddies. Available from: NCBI:BioProject: PRJNA743307 OCE-1259776 is credited since it generated the metal and nutrient data that are part of this dataset. |
| Date received: | 20220706 |
| Start date: | 20140713 |
| End date: | 20140714 |
| Seanames: | |
| West boundary: | -127.317 |
| East boundary: | -124.412 |
| North boundary: | 39.4836 |
| South boundary: | 37.5083 |
| Observation types: | biological, chemical, meteorological, optical, physical |
| Instrument types: | anemometer, flow injection analyzer, fluorometer, mass spectrometer, PCR machine, thermosalinograph |
| Datatypes: | BAROMETRIC PRESSURE, DEPTH - OBSERVATION, FLUORESCENCE, Iron, MANGANESE, nitrate + nitrite content (concentration), phosphate, PHOTOSYNTHETIC ACTIVE RADIATION (PAR), SEA SURFACE TEMPERATURE, silicate, WIND SPEED |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | J. Craig Venter Institute, Old Dominion University, University of California - Santa Cruz |
| Contributing projects: | |
| Platforms: | Melville (318M) |
| Number of observations: | |
| Supplementary information: | Acquisition Description: Sampling location: California Current System 38 N -126 E depth of sampling ~2m Nitrate-nitrite, Phosphate, Silicate: Samples were collected from a trace-metal clean towed-fish system (Bruland et al 2005), filtered through an acid-cleaned, seawater flushed 0.2 um Acropak filter capsule (Pall 500) and analyzed shortly after collection at sea using standard spectrophotometric methods (Parsons 1984) on a Lachat QuickChem 8000 Flow Injection Analysis System. Dissolved iron and manganese samples were collected from a trace-metal clean towed-fish system (Bruland et al., 2005), filtered through an acid-cleaned, seawater flushed 0.2 um Acropak Supor 200 filter capsule (Pall), acidified at sea to pH 1.7-1.8 with quartz-distilled HCl and stored at room temperature until analysis. Once in the lab, samples were buffered to pH 6.0 +/- 0.2 with ammonium acetate and immediately pre-concentrated using Nobias-chelate PA1 chelating resin (concentration factor ~24). Extracts were analyzed on an Element XR High-Resolution Inductively Coupled Plasma Mass Spectrometer (HR ICP-MS) using techniques described initially in Biller and Bruland (2012) with adaptations listed in Parker et al. (2016). Diatom Amplicon Sequencing: Whole seawater was collected from a trace-metal clean towed-fish system (Bruland et al 2005) and filtered onto 3 um polyester filters using a peristaltic pump system. Filters were preserved in RLTPlus buffer and immediately frozen in liquid nitrogen. For long term storage samples were maintained at -80 degrees celsius until extraction. DNA extraction, PCR amplification, and analysis followed that of Chappell et al. 2016 with modification listed in Oliver et al. 2021. Briefly, DNA and RNA were co-extracted using a Qiagen Allprep RNA/DNA mini kit. Diatom 18S was amplified from the DNA using diatom-specific primers modified for Illumina indexing. PCR products were purified, multiplexed using Illumina indices, and sequenced on an Illumina MiSeq desktop sequencer. |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2023-05-20 04:22:58+00 |
| Editdate: | 2023-05-20 04:23:19+00 |