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OAS accession Detail for 0278349
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| accessions_id: | 0278349 | archive |
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| Title: | Abundance and biomass of ciliates from inverted microscope counts from samples taken on R/V Atlantic Explorer cruises AE1102, AE1118, AE1206, AE1219 in the Sargasso Sea, Bermuda Atlantic Time-Series Station in 2011-2012 (Trophic BATS project) (NCEI Accession 0278349) |
| Abstract: | This dataset contains biological, chemical, physical, and survey - biological data collected on R/V Atlantic Explorer during cruises AE1102, AE1118, AE1206, and AE1219 in the North Atlantic Ocean from 2011-02-23 to 2012-07-31. These data include abundance, biomass carbon, depth, and taxon. The instruments used to collect these data include Inverted Microscope and Niskin bottle. These data were collected by Susanne Neuer of Arizona State University as part of the "Plankton Community Composition and Trophic Interactions as Modifiers of Carbon Export in the Sargasso Sea (Trophic BATS)" project and "Ocean Carbon and Biogeochemistry (OCB)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-08-06. The following is the text of the dataset description provided by BCO-DMO: Abundance and biomass of ciliates in the Sargasso Sea from inverted microscope counts. Dataset Description: Abundance (cells per L) and biomass (ng C per L) of ciliates from samples collected during four cruises in the Sargasso Sea during spring and summer 2011-2012. Plot of total_biomass vs. depth. (Generated by BCO-DMO.) Click the image to view a larger version. |
| Date received: | 20190806 |
| Start date: | 20110223 |
| End date: | 20120731 |
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| West boundary: | -64.83 |
| East boundary: | -64.17 |
| North boundary: | 33.48 |
| South boundary: | 30.83 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Water Column Sampling: Water column sampling was performed on four cruises during the spring and the summer of 2011 and 2012 at the Bermuda Atlantic Time-series Study station (31’40°N 64’10°W, BATS) and in the mesoscale eddies found in the surrounding area of the Sargasso Sea. For each cruise, 2 stations were sampled, usually in the center of a mesoscale eddy and at BATS. The edge of the eddy was sample two times, as well. To be able to get a better reproducibility of data, each experiment was replicated. For each experiment, seawater samples were collected pre-dawn (on deck 2:30-4:00, local time) at four different depths within the euphotic zone (20m, 50m, 80m and the Deep Chlorophyll Maximum, DCM). Twenty-one 10L Niskin bottles were attached to a rosette with conductivity, temperature, depth sensors (CTD), and an in vivo fluorometer. This sensor allowed for recording in real time of chlorophyll fluorescence and the DCM for each station. The water that was collected from the 10L Niskin bottles was sampled for abundance and biomass of the plankton community. Microscopy Analyses: Inverted microscopy was used to determine abundance and biomass of planktonic ciliates. Seawater was collected into 200ml amber glass bottles which had previously been supplied with 2.5% of Lugol’s dye (v/v). Samples were stored in the dark and at room temperature onboard ship and in the laboratory at ASU. 100 ml of sample were settled onto settling chambers for 48hr according to the Utermöhl method (Utermöhl, 1931). A Nikon Elipse TE300 inverted microscope was used at 40x magnification to count the entire slide and all the ciliates found were measured and classified based on the classification system introduced by Agatha (2004) and Agatha & Struder-Kypke (2007). Ciliates were classified into 4 standard shapes: prolate spheroid, sphere, cone, cone + half sphere. Biomass calculations were done for each category of organism counted. Biovolume for each group was determined based on size and shape of the organism by approximating the closest geometric shape (Hillebrand et al. 1999) and then converted into units of carbon based on the carbon to volume ratio (Menden-Deuer and Lessard 2000). To determine the carbon biomass of the ciliates, carbon to volume conversion factors were used, as in Putt and Stoecker (1989). The 95% confidence intervals were calculated according to Lund et al. (1958). |
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| Metadata version: | 1 |
| Keydate: | 2023-05-17 12:58:30+00 |
| Editdate: | 2023-05-17 12:59:04+00 |