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OAS accession Detail for 0278314
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| accessions_id: | 0278314 | archive |
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| Title: | Cell abundance estimates of heterotrophic protists by size-class, based on epifluorescence microscopy from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) (NCEI Accession 0278314) |
| Abstract: | This dataset contains biological and survey - biological data collected on R/V Melville during cruise MV1008 in the North Pacific Ocean from 2010-07-04 to 2010-07-19. These data include abundance, depth, and taxon. The instruments used to collect these data include Camera and Inverted Microscope. These data were collected by Michael R. Landry of University of California-San Diego as part of the "Costa Rica Dome FLUx and Zinc Experiments (CRD FLUZiE)" project and "Integrated Marine Biogeochemistry and Ecosystem Research -US (IMBER-US)" and "Ocean Carbon and Biogeochemistry (OCB)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-12-30. The following is the text of the dataset description provided by BCO-DMO: Cell abundance estimates of heterotrophic protists by size-class, based on epifluorescence microscopy. Dataset Description: Cell abundance estimates of heterotrophic protists by size-class, based on epifluorescence microscopy. Samples were collected on the MV1008 cruise in the Costa Rica Dome (CRD) region of the Eastern Tropical Pacific Ocean. |
| Date received: | 20191230 |
| Start date: | 20100704 |
| End date: | 20100719 |
| Seanames: | North Pacific Ocean |
| West boundary: | -92.987 |
| East boundary: | -89.994 |
| North boundary: | 10.3 |
| South boundary: | 8.392 |
| Observation types: | biological, survey - biological |
| Instrument types: | camera, microscope |
| Datatypes: | DEPTH - OBSERVATION, species abundance, TAXONOMIC CODE |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | University of California - San Diego |
| Contributing projects: | |
| Platforms: | Melville (318M) |
| Number of observations: | |
| Supplementary information: | Acquisition Description: Seawater samples (500 mL) for microscopical analysis were gently collected from the CTD and immediately preserved for slide preparation according to a modified protocol of Sherr and Sherr (1993). The samples were first preserved with 260 uL of alkaline Lugol’s solution, immediately followed by 10 mL of buffered formalin and 500 uL of sodium thiosulfate, with gentle mixing between each addition. Preserved samples were shielded from light and left to rest at room temperature for 1 h. After the rest period, 1 mL of proflavin (0.33% w/v) was added and the samples were stored in the dark for an additional hour. Just prior to filtration, the preserved samples were stained with 1 mL of DAPI (0.01 mg mL -1 ) and immediately transferred to the filtration manifold. A 50-mL aliquot (small volume, SV) of the sample was filtered through a 25-mm black polycarbonate filter with 0.8-um pore size, and the remaining 450 mL aliquot (large volume, LV) was filtered through a 25-mm black polycarbonate filter with 8.0-um pores. A 10-mm nylon backing filter was placed under all polycarbonate filters to promote even cell distribution, and filtered the samples under gentle vacuum pressure ( |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2023-05-16 05:04:03+00 |
| Editdate: | 2023-05-16 05:04:46+00 |