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OAS accession Detail for 0278244
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| accessions_id: | 0278244 | archive |
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| Title: | Bacteria cell counts and virus-like particle abundances from Espelandsvegen Fjord, Bergen Norway, May 2017 (NCEI Accession 0278244) |
| Abstract: | This dataset contains biological and survey - biological data collected during deployment MesoHux_2017 from 2017-05-08 to 2017-05-30. These data include abundance and depth. The instruments used to collect these data include Flow Cytometer. These data were collected by Kay D. Bidle and Kimberlee Thamatrakoln of Rutgers University as part of the "Light-dependent regulation of coccolithophore host-virus interactions: mechanistic insights and implications for structuring infection in the surface ocean (Light-dependent host virus interactions)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-01-06. The following is the text of the dataset description provided by BCO-DMO: Bergen MesoHux 2017 Bacteria and Virus Abundance Dataset Description: This dataset includes bacterial and virus abundances using flow cytometry from seawater collected in the Norwegian National Mesocosm Centre at the Espegrend (Espeland) Marine Biological Station near Bergen, Norway, in May 2017. |
| Date received: | 20210106 |
| Start date: | 20170508 |
| End date: | 20170530 |
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| West boundary: | 5.218738 |
| East boundary: | 5.218738 |
| North boundary: | 60.269602 |
| South boundary: | 60.269602 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Environmental Sample Collection Transfer 1 ml of whole seawater to a 2 ml cryovial. Add 20 µl of 25% glutaraldehyde for a final concentration of 0.5%. Incubate at 4°C for 15 min. Flash freeze in liquid N 2 and store at -80°C. Fluorescent DNA staining (for bacterial and viral abundances) Thaw samples. To 20 µl of sample, add 980 µl 1X TE buffer with SYBR Gold (see recipe below) Heat to 80°C for 10 min in the dark Cool at RT for 10 min in the dark Analyze via flow cytometry Analysis (for bacterial and viral abundances) Samples are analyzed on Influx Model 209S Mariner flow cytometer using BD Sortware (BD Biosciences). An initial Forward Scatter (FSC) vs Side Scatter (SSC) configuration is determined using Molecular Probes Flow Cytometry Sub-micron particles size reference kit (Cat#F13839) consisting of 0.02, 0.1, 0.5, 1.0 and 2.0 µm fluorescent beads. A gating hierarchy is established using both beads and previously determined virus and bacteria populations as reference (Sybr Gold Fluorescence versus SSC cytogram). Samples are analyzed using a 488 nm laser for excitation and a minimum trigger threshold is established using 542/15 nm (SYBR Gold) emission. Flow rates are determined using the volumetric method. Collected data are analyzed using FlowJo v9.9.6. Virus Like Particles (VLPs) are differentiated from bacteria using the gating hierarchy established at time of collection. TE buffer with SYBR Gold recipe 1X TE (for 100 mls) 1 ml of 1M Tris, pH 8.0 1 ml of 0.5 mM EDTA 98 mls MQ water Store 4ºC 1X TE + SYBR Gold (for 10 mls) 1. Filter 10 mls 1 TE buffer, 0.22 μm filter 2. 1:20,000 dilution of SYBR Gold stock (Molecular Probes) (0.5 μl stock to 10 mls TE buffer) |
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| Metadata version: | 1 |
| Keydate: | 2023-05-15 04:29:25+00 |
| Editdate: | 2023-05-15 04:29:46+00 |