The Ocean Archive System searches our original datasets as they were submitted to us, not individual points or profiles. If you want to search and retrieve ocean profiles in a common format, or objectively analyzed fields, your better option may be to use one of our project applications. See: Access Data
OAS accession Detail for 0278230
| << previous | |revision: 1 |
| accessions_id: | 0278230 | archive |
|---|---|
| Title: | Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) (NCEI Accession 0278230) |
| Abstract: | This dataset contains biological and survey - biological data collected on R/V Melville during cruise MV1008 in the North Pacific Ocean from 2010-06-24 to 2010-07-24. These data include depth, prochlorococcus abundance, and synechococcus abundance. The instruments used to collect these data include Flow Cytometer and Niskin bottle. These data were collected by Michael R. Landry of University of California-San Diego as part of the "Costa Rica Dome FLUx and Zinc Experiments (CRD FLUZiE)" project and "Integrated Marine Biogeochemistry and Ecosystem Research -US (IMBER-US)" and "Ocean Carbon and Biogeochemistry (OCB)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-12-27. The following is the text of the dataset description provided by BCO-DMO: Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis). Dataset Description: Picophytoplankton abundances from preserved (0.5% paraformaldehyde) frozen samples run on a Beckman-Coulter Altra flow cytometer after staining with Hoechst 33342 DNA stain and excitation with 200 mW UV and 1W 488 nm laser light. Cell populations include Prochlorococcus , Synechococcus, and Picophytoeukaryotes. Samples were collected on the MV1008 cruise in the Costa Rica Dome (CRD) region of the Eastern Tropical Pacific Ocean. |
| Date received: | 20191227 |
| Start date: | 20100624 |
| End date: | 20100724 |
| Seanames: | |
| West boundary: | -92.987 |
| East boundary: | -86.735 |
| North boundary: | 10.3 |
| South boundary: | 6.622 |
| Observation types: | |
| Instrument types: | |
| Datatypes: | |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | |
| Contributing projects: | |
| Platforms: | |
| Number of observations: | |
| Supplementary information: | Acquisition Description: Samples were collected from the CTD-Rosette PVC Niskin bottles, then preserved with paraformaldehyde (0.5% final concentration). Preserved samples were then flash frozen in liquid nitrogen, and subsequently stored at -80°C until analysis on-shore in batches. Upon thawing, samples were stained with Hoechst 33342 DNA stain (1 ug/mL final concentration), incubated at room temperature in the dark for 1 h, then analyzed using a Beckman-Coulter Altra flow cytometer, equipped with one 488 nm 1W laser and one UV 200 mM laser for excitation. Samples (100 ul) were delivered quantitatively to the instrument using a Harvard Apparatus syringe pump, at a rate of 50 ul per minute. Signals from the DNA (450±40 nm), Chlorophyll (680±20 nm), Phycoerythrin (575±20 nm), and the forward and side scatter detectors were used to delineate Prochlorococcus , Synechococcus , and Picophytoeukaryotes. Calibration beads (0.5 um and 1.0 um yellow-green and 0.5 um UV) were used to normalize cellular fluorescence values and assure optimal instrument settings. Raw data files (listmode) were processed in FlowJo software (Treestar Inc.). |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2023-05-15 04:04:48+00 |
| Editdate: | 2023-05-15 04:05:36+00 |