The Ocean Archive System searches our original datasets as they were submitted to us, not individual points or profiles. If you want to search and retrieve ocean profiles in a common format, or objectively analyzed fields, your better option may be to use one of our project applications. See: Access Data
OAS accession Detail for 0278153
| << previous | |revision: 1 |
| accessions_id: | 0278153 | archive |
|---|---|
| Title: | Diel series of pico-cyanobacteria concentration and cell properties from the RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010 (NCEI Accession 0278153) |
| Abstract: | This dataset contains biological and optical data collected on R/V Cape Hatteras during cruises CH0409 and CH0510 from 2009-05-26 to 2010-05-31. These data include depth and fluorescence. The instruments used to collect these data include Flow Cytometer and Niskin bottle. These data were collected by Dr Brian Binder of University of Georgia as part of the "Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach (Picophytoplankton_Regulation)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-06-11. The following is the text of the dataset description provided by BCO-DMO: Diel series of pico-cyanobacteria concentration and cell properties. Dataset Description: Diel series of pico-cyanobacteria concentration and cell properties. These data were published in Hynes et al., 2015 and Rhodes K.L., 2009 Other relevant publication: Binder et al., 1996 |
| Date received: | 20190611 |
| Start date: | 20090526 |
| End date: | 20100531 |
| Seanames: | |
| West boundary: | -72.8769 |
| East boundary: | -71.8634 |
| North boundary: | 30.9082 |
| South boundary: | 30.1464 |
| Observation types: | |
| Instrument types: | |
| Datatypes: | |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | |
| Contributing projects: | |
| Platforms: | |
| Number of observations: | |
| Supplementary information: | Acquisition Description: Samples were taken using a rosette of Niskin bottles, fixed with freshly titrated paraformaldehyde (pH 7.4–8.1, 0.1% final concentration), held in the dark for 10 min, frozen in liquid nitrogen, and stored in a -80 deg C freezer (CH0409 samples) or in liquid nitrogen (CH0510 samples) until analysis. Preserved samples were analyzed by dual beam flow cytometry on a modified Coulter-EPICS 753 flow cytometer (Binder et al. 1996). Samples were chosen in random order, defrosted in a 30°C water bath (just long enough to melt, ~5 min), and stained with the DNA-specific stain Hoechst 33342 (0.5 ug mL-1 final concentration) (Invitrogen, Carlsbad, California) for a minimum of 20 min in the dark. Prior to analysis, polystyrene fluorescent beads (0.5 um and 1.0 um diameter Flow CheckVR ; Polysciences, Washington, Pennsylvania), were added to each sample, and used to normalize cellular light scatter and red (chlorophyll-derived) fluorescence. Samples were run at an infusion rate of 10 uL min-1 for 10–50 min, depending on cell abundance within the sample. Mean red fluorescence and forward angle light scatter for each cell type in each sample are linearized and normalized to 1.0 um and 0.5 um diameter beads (see above), respectively. Thus the measurements are relative, and are only meaningful for comparisons of cellular properties within this data set. |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2023-05-13 04:46:00+00 |
| Editdate: | 2023-05-13 04:46:19+00 |