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OAS accession Detail for 0278146
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accessions_id: | 0278146 | archive |
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Title: | Presence/absence of taxa detected with the Phylochip in a sediment core collected in the Ulleng Basin in 2011 (NCEI Accession 0278146) |
Abstract: | This dataset contains biological and survey - biological data collected during deployment Ulleung_Basin_Core in the Japan Sea from 2011-01-01 to 2011-12-31. These data include family, order, and phylum. The instruments used to collect these data include Piston Corer. These data were collected by Brandon Briggs and Frederick Colwell of Oregon State University as part of the "The Effect of Methane on Microbial Community Distributions in the Ulleung Basin (Methane_Microbes)" project and "Center for Dark Energy Biosphere Investigations (C-DEBI)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-05-20. The following is the text of the dataset description provided by BCO-DMO: Dataset Description: Presence/ absence of taxa detected with the Phylochip. |
Date received: | 20210520 |
Start date: | 20110101 |
End date: | 20111231 |
Seanames: | Japan Sea |
West boundary: | 131 |
East boundary: | 131 |
North boundary: | 37 |
South boundary: | 37 |
Observation types: | biological, survey - biological |
Instrument types: | corer |
Datatypes: | SPECIES IDENTIFICATION |
Submitter: | |
Submitting institution: | Biological and Chemical Oceanography Data Management Office |
Collecting institutions: | Oregon State University |
Contributing projects: | |
Platforms: | |
Number of observations: | |
Supplementary information: | Acquisition Description: In summary, sediment cores were collected using a Furor Hydraulic Piston Corer following the Integrated Ocean Drilling Program (IODP) guidelines for obtaining high quality microbiology cores. Aboard ship, whole round cores were subcored by paring the outer two cm of sediment with an alcohol and flame sterilized chisel. The sub-cores were placed into sterile Whirl Pak Bags and stored at -80C. DNA was extracted from 10g of sediment using a PowerMax Soil DNA extraction kit. DNA was amplified using 27F and 1492R (bacterial) or 4F and 1492R (archaeal) universal primers. 150ng of bacterial and 50ng of archaeal amplicons were hybridized to the PhyloChip array. |
Availability date: | |
Metadata version: | 1 |
Keydate: | 2023-05-13 04:36:12+00 |
Editdate: | 2023-05-13 04:36:48+00 |