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OAS accession Detail for 0278145
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| accessions_id: | 0278145 | archive |
|---|---|
| Title: | Single amplified genomes (SAGs) of microbial cells isolated from deep-sea hydrothermal vent 'Crab Spa', East Pacific Rise, Pacific Ocean from R/V Atlantis AT15-38 and AT26-10, 2008 and 2014 (Microbial Communities at Deep-Sea Vents project) (NCEI Accession 0278145) |
| Abstract: | This dataset contains biological data collected on R/V Atlantis during cruises AT15-38 and AT26-10 in the North Pacific Ocean from 2008-10-01 to 2014-01-15. These data include taxon and taxon_code. The instruments used to collect these data include Automated DNA Sequencer, Isobaric Gas-Tight Sampler, and ROV Jason. These data were collected by Dr Ramunas Stepanauskas of Bigelow Laboratory for Ocean Sciences as part of the "An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents (Microbial Communities at Deep-Sea Vents)" project and "Center for Dark Energy Biosphere Investigations (C-DEBI)" and "Dimensions of Biodiversity (Dimensions of Biodiversity)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-04-07. The following is the text of the dataset description provided by BCO-DMO: Single amplified genomes (SAGs) of microbial cells isolated from Crab Spa, East Pacific Rise Dataset Description: The accession links are not yet public. They will be archived at the Integrated Microbial Genomes (IMG), at the Joint Genome Inst. (JGI), US Dept. of Energy (DOE). Please contact the PI for further details. |
| Date received: | 20210407 |
| Start date: | 20081001 |
| End date: | 20140115 |
| Seanames: | North Pacific Ocean |
| West boundary: | -104.291 |
| East boundary: | -104.291 |
| North boundary: | 9.84 |
| South boundary: | 9.84 |
| Observation types: | biological |
| Instrument types: | |
| Datatypes: | TAXONOMIC CODE |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | Bigelow Laboratory for Ocean Sciences |
| Contributing projects: | |
| Platforms: | Atlantis (33AT) |
| Number of observations: | |
| Supplementary information: | Acquisition Description: AT15-38: Samples for SAGs were obtained using the so-called 'titanium major samplers' (von Damm et al, 1985). Replicate samples of 1 ml aliquots of water were cryopreserved with 6% glycine betaine (Sigma) or 15% glycerol and stored at -80 ºC for the ”Single Cell” aliquot. AT26-10: Background samples were obtained from the IGTs, in which the incubations were carried out. Just in this case, an aliquot was removed after sample retrieval and before starting the incubation. During AT26-10, samples were preserved with Gly-TE and stored at -80. Cells were sorted, identified and sequenced by the Bigelow Laboratory Single Cell Genomics FacilityCenter (SCGC), following SCGCthe facilities’s standard practices: SCGC_Services_Description.pdf |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2023-05-13 04:34:49+00 |
| Editdate: | 2023-05-13 04:35:26+00 |