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OAS accession Detail for 0278094
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| accessions_id: | 0278094 | archive |
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| Title: | Doliolid abundance, carbon and nitrogen content, chlorophyll-a, temperature, salinity, and depth from CTD casts from 25 RV Savannah cruises at the South Atlantic Bight, 2015-2017 (NCEI Accession 0278094) |
| Abstract: | This dataset contains biological, chemical, physical, and survey - biological data collected on R/V Savannah during cruises SAV-15-10, SAV-15-19, SAV-15-31, SAV-16-02, SAV-16-04, SAV-16-08, SAV-16-11, SAV-16-15, SAV-16-20, SAV-16-23, SAV-16-27, SAV-16-33, SAV-16-34, SAV-16-37, SAV-16-40, SAV-17-01, SAV-17-03, SAV-17-04, SAV-17-06, SAV-17-11, SAV-17-14, SAV-17-19, SAV-17-22, SAV-17-23, and SAV-17-25 in the North Atlantic Ocean from 2015-05-19 to 2015-12-02. These data include abundance, chlorophyll a, d15N measured in biota, depth, particulate organic Carbon (POC), particulate organic nitrogen, salinity calculated from CTD primary sensors, total phaeopigment, and water temperature. The instruments used to collect these data include CTD profiler, Elemental Analyzer, Isotope-ratio Mass Spectrometer, Niskin bottle, and Turner Designs Fluorometer -10-AU. These data were collected by Dr Deidre M. Gibson of Hampton University and Marc E. Frischer of Skidaway Institute of Oceanography as part of the "The cryptic diet of the globally significant pelagic tunicate Dolioletta gegenbauri (Uljanin, 1884.) (Doliolid Diet)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-06-10. The following is the text of the dataset description provided by BCO-DMO: Nutrients and chlorophyll-a from CTD casts 2015-2017 Dataset Description: This dataset includes Doliolid abundance, carbon and nitrogen content, depth, temperature, salinity and in situ chlorophyll a from 25 cruises on the R/V Savannah at the South Atlantic Bight, Mid-Continental Shelf, (31 N 80 W) during 2015-2017. From the bottle samples, the following parameters were also measured: - Average chemical extracted size fractionated total and > 8 um chlorophyll a and phaeophytin (mg m-3) - Total and size fractionated (- Total and size fractionated (- Total and size fractionated (- Total and size fractionated (- Average delta13C (per mil vs PDB standard) and delta15N (per mil vs air N2) of starved D. gegenbauri zooids. - D. gegenbauri abundance (individuals m-3) |
| Date received: | 20190610 |
| Start date: | 20150519 |
| End date: | 20151202 |
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| West boundary: | -81.18 |
| East boundary: | -80.097 |
| North boundary: | 31.93 |
| South boundary: | 29.945 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Depth, Temperature, salinity and in situ chlorophyll a salinity were recorded during the cruise from the real-time CTD data feed, recorded in log books. Complete CTD cast records are included as an independent dataset associated with this deployment. Surface values were recorded during the period when the CTD rosette entered the water and were logged once values had stabilized. Near bottom values were logged when CTD had reached its maximum deployment depth and values stabilized. Extracted chlorophyll a and phaeophytin values were determined following overnight extraction in 90% acetone essentially as described by Parsons et al. (1984) after filtering 100 – 250 ml of water sample through either 0.2 microns (total) or 8 microns (> 8 microns) polycarbonate membrane filters. Filtrations were conducted in a darkened room. Filtration times were kept to less than 20 minutes per sample and less than 10 mm Hg vacuum. Following filtration, filters were placed directly into clean extraction tubes and stored frozen at -20ºC. Filters were extracted and analyzed within one week of collection. Fluorescence was measured using a 10AU Turner Designs fluorometer that was calibrated regularly using a chlorophyll standard solution (Sigma Chl std cat #C6144 – 1 mg). Standard curves were prepared as a 12-point dilution series from 0.05 – 500 micrograms L-1. Water particulate organic matter (POC and PON) concentrations and isotopic composition were estimated from water samples (200 – 500 ml) filtered through pre-combusted Whatman GF/F filters. Filters were dried and stored at 60 deg.C until analysis. Organic matter (C and N) isotopic composition of animals was also estimated. Individual animals were placed in 8x10 mm tin cups (Elemental Microanalysis) with a minimum of fluid, and dried at 60ºC for at least 48 hours prior to analysis. Samples were stored in 48-well plates at 60ºC prior to final preparation. Sample analysis for %C, % N, d13C and d15N were performed on a ThermoFisher Delta V plus isotope ratio mass spectrometer interfaced to a Thermo Flash Elemental analyzer (Fry 1992). Isotopic and elemental composition calibrations were performed using commercially available powdered chitin (Sigma Chemical), which in turn had been calibrated against a variety of international (USGS 40 Glutamic Acid) and commercial (Elemental Microanalysis B2151 and B2155) isotopic and compositional standards. Chitin standards were run at the start of each analysis across the range of C amounts anticipated to be present in the samples, and every 10 samples as a drift check. |
| Availability date: | |
| Metadata version: | 2 |
| Keydate: | 2023-05-12 04:31:55+00 |
| Editdate: | 2024-04-17 04:40:36+00 |