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OAS accession Detail for 0277719
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| accessions_id: | 0277719 | archive |
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| Title: | Single colony metaproteomes of Trichodesmium from samples collected in North Atlantic surface waters during the R/V Atlantis cruise AT39-05 in March of 2018 (NCEI Accession 0277719) |
| Abstract: | This dataset contains biological and survey - biological data collected on R/V Atlantis during cruise AT39-05 on 2018-03-11. These data include abundance and taxon. The instruments used to collect these data include Mass Spectrometer. These data were collected by Mak A. Saito of Woods Hole Oceanographic Institution as part of the "Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean (TriCoLim)", "Collaborative Research: Evolutionary, biochemical and biogeochemical responses of marine cyanobacteria to warming and iron limitation interactions (Cyanobacteria Warming Responses)", "Marine Microbial Investigator Award: Investigator Mak Saito (MM Saito)", and "New technology for high resolution analysis of proteins and other organic materials produced by marine microorganisms (MM Proteins and Organics Tech)" projects and "Marine Microbiology Initiative (MMI)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-10-08. The following is the text of the dataset description provided by BCO-DMO: Dataset Description: Single colony metaproteomes of Trichodesmium from samples collected in North Atlantic surface waters during the R/V Atlantis cruise AT39-05 in March of 2018. These data were published in Held et al. (2020). The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [1] partner repository with the dataset identifier PXD016330 and 10.6019/PXD016330 but are not yet public. * Project Name: Trichodesmium single colony metaproteomes field station Tricolim_19 * Project accession: PXD016330 * Project https://doi.org/10.6019/PXD016330 |
| Date received: | 20201008 |
| Start date: | 20180311 |
| End date: | 20180311 |
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| West boundary: | 65.22 |
| East boundary: | 65.22 |
| North boundary: | 17.02 |
| South boundary: | 17.02 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: A 200mm plankton net was deployed to ~20m depth, then recovered to just below surface, repeating five times. Trichodesmium colonies were hand-picked into 0.2mm filtered surface seawater, rinsed twice in 0.2mm filtered surface seawater, and decanted onto a 0.2-4mm Supor filter (indicated in sample provenance table, see dataset https://www.bco-dmo.org/dataset/787093). Proteins were extracted and trypsin digested in-gel following Saito et al., 2014 (Science) with the following modifications: organic precipitation was skipped, benzonase nuclease treatment was performed instead to dissolve DNA/RNA material. Peptides were analyzed by LC-MS/MS in data discovery mode on a Thermo Orbitrap Fusion. Full details will be reported in Held et al. (in prep) Location: North Atlantic surface waters 65.22 W 17.02 N |
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| Metadata version: | 2 |
| Keydate: | 2023-04-20 04:44:13+00 |
| Editdate: | 2023-06-26 22:01:11+00 |