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OAS accession Detail for 0277469
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| accessions_id: | 0277469 | archive |
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| Title: | Rates of primary and bacterial production, and chlorophyll concentrations measured experimentally under ambient and elevated pCO2 (750 or 1100 µatm) from Hawaii Ocean Time-series near Station ALOHA from 2010-2011 (NCEI Accession 0277469) |
| Abstract: | This dataset contains biological and chemical data collected on R/V Kilo Moana during cruises KM1015, KM1016, KM1017, KM1019, KM1101, KM1108, KM1110, KM1113, and KM1219 in the North Pacific Ocean from 2010-08-07 to 2012-09-09. These data include chlorophyll a, depth, dissolved inorganic Carbon, leucine incorporation rate, primary production, and total alkalinity. The instruments used to collect these data include CTD profiler and Liquid Scintillation Counter. These data were collected by Dr Ricardo Letelier of Oregon State University and Matthew J. Church of University of Hawaii as part of the "Oceanic diazotroph community structure and activities in a high carbon dioxide world (DIAZOTROPHS-CO2)" project and "Ocean Carbon and Biogeochemistry (OCB)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23. The following is the text of the dataset description provided by BCO-DMO: bubbled pCO2 perturbation experiments Dataset Description: This data was used in Viviani et al (2018). For related research of experimental work done on some of the same cruises and drawn from some of the same experiments but reporting different parameters, see Bottjer et al (2014). |
| Date received: | 20230123 |
| Start date: | 20100807 |
| End date: | 20120909 |
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| West boundary: | -158.312 |
| East boundary: | -157.968 |
| North boundary: | 24.004 |
| South boundary: | 21.447 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Rates of primary production were assessed using the 14C-bicarbonate incorporation technique. Rates of bacterial production were assessed using incorporation of 3H-leucine. Whole near-surface seawater was collected into acid-washed 20 L carboys. Control carboys were bubbled with air; treatment carboys were bubbled with a mixture of air and CO2, to increase the pCO2 to either ~750 or 1100 µatm. Sampling of each time point was conducted before dawn, experiments lasted between 2 and 5 days. Water from each carboy was subsampled into acid washed 500 mL polycarbonate bottles for primary production rate measurements. To each bottle, was then added ~1.85 MBq 14C-bicarbonate. Water from each carboy was also collected in an opaque polyethylene amber bottles and then subsampled into six 1.5 mL microcentrifuge tubes for bacterial production rate measurements. Each tube was then inoculated with 3H-leucine to a final concentration of 20 nmol L-1. Three tubes from each carboy were incubated in the dark (in a opaque cloth bag) and three in the light. Time zero blanks were immediately subsampled from each amber bottle, by aliquoting 1.5 mL of seawater into 2 mL microcentrifuge tubes each containing 100 µL of 100% TCA. Following addition of radioactive substrates, the primary production bottles and bacterial production tubes were placed in shaded (~50% irradiance) surface seawater-cooled incubators for the duration of the photoperiod. After sunset, the total radioactivity added to each primary production sample bottle was determined by subsampling 250 µL aliquots of seawater into scintillation vials containing 500 µL of β-phenylethylamine. 100 mL from each 500 mL sample bottle was filtered at low vacuum ( After sunset, 100 µL of 100% TCA was added to each microcentrifuge tube. The microcentrifuge tubes were frozen (-20°C) for subsequent processing, following the procedures described in Smith and Azam 1992. Samples for the determination of dissolved inorganic carbon and total alkalinity were collected from each carboy and analyzed according to the protocols of the Hawaii Ocean Time-series (Dore et al. 2009; Winn et al. 1998). DIC and TA samples were collected into precombusted 300 mL borosilicate bottles. Care was taken to avoid introduction of air bubbles into samples during filling; bottles were allowed to overflow three times during filling. Once filled, samples were immediately fixed with 100 µL of a saturated solution of mercuric chloride; bottles were capped with a grease seal, and stored in the dark for later analysis. Samples for measurement of fluorometric chlorophyll a were collected according to the protocols of the Hawaii Ocean Time-series; analysis was performed following Letelier et al. (1996). |
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| Metadata version: | 1 |
| Keydate: | 2023-04-04 05:32:23+00 |
| Editdate: | 2023-04-04 05:33:11+00 |