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OAS accession Detail for 0277327
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| accessions_id: | 0277327 | archive |
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| Title: | Survival, length, and growth responses of M. menidia offspring from different females exposed to contrasting CO2 environments on 2017-11-14 (NCEI Accession 0277327) |
| Abstract: | This dataset contains biological, chemical, and survey - biological data collected at lab Avery_Point during deployment AP_Rankin on 2017-11-14. These data include growth, pCO2, pH, and species. The instruments used to collect these data include Automatic titrator and pH Sensor. These data were collected by Dr Janet Nye of Stony Brook University - SoMAS and Dr Hannes Baumann of University of Connecticut as part of the "Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish (HYPOA)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23. The following is the text of the dataset description provided by BCO-DMO: Survival, length, and growth responses of M. menidia offspring from different females exposed to contrasting CO2 environments. Dataset Description: Wild-caught M. menidia adults were spawned to test whether offspring from different mothers differ in their average survival and size responses to elevated CO2 conditions. The experiment quantified three related survival and three size traits for each replicate, female, and CO2 treatment: embryo survival (fertilization to 1 dph), larval survival (1 to 16 dph), and overall survival (fertilization to 16 dph); and size (SL) at hatch (1 dph), SL at 16 dph , and larval growth rate (GR = (SL16dph – SL1dph)/15). These data are associated with the corresponding paper: Snyder, J.T.*, Murray, C.S.*, and Baumann, H. (2017) Potential for maternal effects on offspring CO2-sensitivities in a coastal marine fish. Journal of Experimental Marine Biology and Ecology (in press). Other datasets related to this paper: Fatty acid profiles of M. menidia females and their unfertilized eggs. |
| Date received: | 20230123 |
| Start date: | 20171114 |
| End date: | 20171114 |
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| West boundary: | -72.02 |
| East boundary: | -72.02 |
| North boundary: | 41.32 |
| South boundary: | 41.32 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Methodology from Snyder, J.T.*, Murray, C.S.*, and Baumann, H. (2017) Potential for maternal effects on offspring CO2-sensitivities in a coastal marine fish. Journal of Experimental Marine Biology and Ecology (in press). Five randomly selected females were strip-spawned onto cutout sections of window screen (1-mm mesh) that were placed into separate seawater-filled spawning dishes (Murray et al., 2014). To ensure full fertilization success and randomize potential paternal effects, eggs were fertilized with a mixture of milt from 22 males, thus producing full-sib and maternal half-sib embryos from each female. Adults were measured for total length (TL; mean TLmale = 9.14 cm, mean TLfemale = 10.4 cm) and frozen for later analysis of FA. Mesh screens with attached embryos were subsequently cut into smaller sections to allow precise enumeration, and within 2-hr post-fertilization 100 embryos were placed into each of three replicate rearing containers (20 L) per female and CO2 treatment (i.e., 600 embryos for each of five females, 3 × 100 in ambient and 3 × 100 in acidified treatments). Rearing containers were filled with 1-um filtered, UV-sterilized seawater (~30 psu) from Long Island Sound and placed in temperature-controlled water baths set to 24 deg C, the known thermal optimum for survival and growth in this species (Middaugh et al., 1987). Offspring were reared for 24 d post fertilization under a 15h light:9h dark lighting regime. After hatch, larvae were fed ad libitum rations of newly hatched brine shrimp nauplii Artemia salina (brineshrimpdirect.com), and 50% of water was replaced every 5 d to ensure safe ammonia levels ( CO2 regime: Offspring were reared at ambient (~ 400 uatm, pHNBS = 8.18) and acidified CO2 conditions (~2,300 uatm, pHNBS = 7.50). The higher value was set to a level commonly used in OA research (consistent with projections of future pCO2 values for open oceans over in the next 200 yr (IPCC, 2007)) and represents current conditions experienced during seasonal extremes by this species in nature (Murray et al., 2014). Ambient conditions were achieved by bubbling partially CO2-stripped air into each rearing container, thereby offsetting metabolic CO2 accumulation. Acidified conditions were achieved via gas proportioners (Cole Parmer®) that mixed CO2 stripped air with 100% bone-dry CO2 delivered to the bottom of each rearing container via air stones. Target pH and temperature were monitored daily via a handheld pH probe (Hach® HQ40d portable meter with a PHC201 standard pH-probe) calibrated regularly via two-point National Bureau of Standards (NBS) pH buffers (electronic supplementary material, Fig.S1). To characterize actual pCO2 levels and related water chemistry parameters, water was sampled from four randomly chosen rearing containers per treatment three times over the course of the experiment and immediately measured for total alkalinity (AT) via endpoint titration (Mettler Toledo™ G20 Potentiometric Titrator). The instrument has previously been shown to quantify AT in Dr. Andrew Dickson’s reference material (batch 147, AT= 2231.39 umol kg seawater-1) with an average error of 0.6%. Actual levels of total dissolved inorganic carbon (CT), partial pressure of CO2 (pCO2), fugacity of CO2 (fCO2), and carbonate ion concentration were calculated in CO2SYS (http://cdiac.ornl.gov/ftp/co2sys) based on measured AT, pH (NBS), temperature, and salinity using K1 and K2 constants from Mehrbach et al. (1973) refit by Dickson and Millero (1987) and Dickson (1990) for KHSO4 (Table 1). |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2023-04-01 14:41:41+00 |
| Editdate: | 2023-04-01 14:42:21+00 |