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OAS accession Detail for 0277298
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| accessions_id: | 0277298 | archive |
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| Title: | Flow cytometry results for naupliar grazing laboratory experiments conducted from 2012-2013 (EAGER: Copepod nauplii project) (NCEI Accession 0277298) |
| Abstract: | This dataset contains biological and survey - biological data collected at lab UHawaii_SOEST during deployment Goetze_2012-2013 from 2013-05-29 to 2013-06-05. These data include abundance. The instruments used to collect these data include Flow Cytometer. These data were collected by Dr Petra Lenz, Erica Goetze, and Karen E. Selph of University of Hawaii at Manoa as part of the "New molecular methods for studying copepod nauplii in the field (EAGER: Copepod nauplii)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23. The following is the text of the dataset description provided by BCO-DMO: Flow cytometry results for naupliar grazing experiments (laboratory) Dataset Description: These data report initial and final measurements of prey abundances in bottle incubation experiments to measure naupliar grazing. |
| Date received: | 20230123 |
| Start date: | 20130529 |
| End date: | 20130605 |
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| West boundary: | -157.7797 |
| East boundary: | -157.7797 |
| North boundary: | 21.4322 |
| South boundary: | 21.4322 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: All samples were collected from surface water in Southern Kaneohe Bay, Oahu, Hawaii (21°25’56.7”N, 157°46’47.1”W). Pro, Syn, Peuk, PE+Peuk, HBact data: These data were collected and analyzed by flow cytometry as described in Selph et al. (2011). Briefly, samples were preserved (0.05% paraformaldehyde, final concentration), and frozen to -80°C within 1 h. Batches of samples were thawed, stained with Hoechst 34442 (1 µg/mL final concentration) and analyzed on a flow cytometer (Beckman Coulter Altra) with dual laser excitation (UV range and 488 nm, 200 mW and 1 W, respectively), mated to a Harvard Apparatus syringe pump for quantitative delivery (100 µl at 50 µl min -1 ). Resulting listmode files were analyzed off-line using FlowJo software (Treestar). Populations determined, based on red fluorescence (chlorophyll, 680±20 nm), orange fluorescence (phycoerythrin, 575±20 nm), blue fluorescence (DNA, 450±40 nm), and light scatter signals (forward and 90°). Distinguishable populations were Prochlorococcus , Synechococcus , Photosynthetic Eukaryotes (PEUK), High phycoerythrin-containing PEUK (PE+Peuk), and non-pigmented prokaryotes (a.k.a. heterotrophic bacteria, HBACT). |
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| Metadata version: | 1 |
| Keydate: | 2023-03-30 22:41:26+00 |
| Editdate: | 2023-03-30 22:41:41+00 |