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OAS accession Detail for 0277265
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| accessions_id: | 0277265 | archive |
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| Title: | Compound-specific nitrogen isotopes from sperm whale skin tissue from the UC-Santa Cruz labs of P. Koch and M. McCarthy (Sperm Whale SI Ratios project) from 1972-12-01 to 2005-07-31 (NCEI Accession 0277265) |
| Abstract: | This dataset contains chemical data collected at UCSC during deployment lab_UCSC_Koch in the North Pacific Ocean from 1972-12-01 to 2005-07-31. These data include d15N measured in biota. The instruments used to collect these data include Isotope-ratio Mass Spectrometer. These data were collected by Matthew D. McCarthy and Paul L. Koch of University of California-Santa Cruz as part of the "A novel approach for evaluating temporal and spatial changes in trophic structure of the mesopelagic eastern Pacific (Sperm Whale SI Ratios)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23. The following is the text of the dataset description provided by BCO-DMO: Compound-specific nitrogen isotopes from sperm whale skin tissue Dataset Description: Acquisition Description: Materials and methods for analysis are described in detail in the Ruiz_Cooly et al (2014). Skin tissue samples with enough material (3.5 mg) were selected for compound specific isotope analysis of amino acids (CSIA-AA). Data from 12 samples were obtained for individual amino acid (AA) d15N values, and 9 samples for d13C values. The Southwest Fisheries Science Center/Pacific Islands Fisheries Science Center Institutional Animal Care and Use Committee (IACUC) approved the original animal work that produced the samples. Sex was determined genetically using qPCR sexing assay by the PRD-Genetic Lab at NOAA. These samples consisted of 5 females, 2 males and 2 unidentified individuals possibly corresponding to females or juvenile males. Large adult males were not included. The relative pattern of AA d15N and d13C values was highly consistent with past work from other organisms and tissues [1-3]. We grouped data as source- or trophic-AAs for d15N values, and essential- or non-essential-AAs for d13C values to increase power in the analysis and evaluate temporal variation. We calculated average values for each AA group. Regression analyses were conducted to evaluate linear relationship between time and each isotopic tracer for both bulk and individual-AA d15N and d13C values. We hydrolyzed and prepared approximately 3.5 mg of skin as well as a control (Cyanno; bacteria tissue) [1] to quantify d15N values from source- and trophic-AAs and d13C values from essential- and non-essential-AAs. All derivatives were injected with an AA control, N-leucine, to verify accuracy during each run, and analyzed via gas chromatography-IRMS to obtain d15N and d13C values from individual AAs. Each sample was run 3–4 times to maximize accuracy among chromatograms. The associated analytical error among replicates typically Cited References: 1. McCarthy, et al, (2007) 2. Popp, et al. (2007 3. Sherwood, et al (2011) |
| Date received: | 20230123 |
| Start date: | 19721201 |
| End date: | 20050731 |
| Seanames: | North Pacific Ocean |
| West boundary: | -128.2 |
| East boundary: | -117.35 |
| North boundary: | 46.85 |
| South boundary: | 32.47 |
| Observation types: | chemical |
| Instrument types: | mass spectrometer |
| Datatypes: | DELTA NITROGEN-15 |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | University of California - Santa Cruz |
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| Number of observations: | |
| Supplementary information: | |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2023-03-30 15:11:25+00 |
| Editdate: | 2023-03-30 15:12:07+00 |