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OAS accession Detail for 0256657
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Title: Zooplankton biomass and density collected from R/V Laurentian and R/V Lake Guardian in Lake Huron in the Great Lakes region from 2007-04-14 to 2017-09-19 (NCEI Accession 0256657)
Abstract: NOAA Great Lakes Environmental Research Laboratory (GLERL) samples and studies the ecosystem of the lower food web of the Laurentian Great Lakes. This collection contains zooplankton biomass and density data at 21 stations during the open water seasons of 2007, 2012, and 2017 in Lake Huron. This collection of monitoring data was in coordination with the Great Lakes Cooperative Science and Monitoring Initiative (CSMI). Zooplankton density is given in the number of organisms per cubic meter and biomass is given in milligrams per cubic meter. File formats included in this data package are .csv and .jpg.
Date received: 20220701
Start date: 20070414
End date: 20170919
Seanames: Great Lakes
West boundary: -83.86
East boundary: -82.23
North boundary: 45
South boundary: 43.6
Observation types: laboratory analyses
Instrument types: net - zooplankton net
Datatypes: ZOOPLANKTON BIOMASS, ZOOPLANKTON DENSITIES
Submitter: Mason, Lacey
Submitting institution: US DOC; NOAA; OAR; Great Lakes Environmental Research Laboratory
Collecting institutions: US DOC; NOAA; OAR; Great Lakes Environmental Research Laboratory
Contributing projects:
Platforms: Lake Guardian (pid: 13681), LAURENTIAN (33LA)
Number of observations:
Supplementary information: Methods for Lake Huron zooplankton

Zooplankton were collected with a 0.5 meter diameter, 2-m long, 153 or 64 µm mesh net. The net was vertically towed through the water column at a speed of 0.5 m s-1 from 1 to 2 meters above the bottom to the surface. For partial water column tows, to collect separately the epilimnion, metalimnion and hypolimnion, a messenger actuated choke-off vertical opening/closing net (0.5m diameter, 2m-long, 153 or 64 µm mesh) was used to obtain the zooplankton samples. The net was washed thoroughly, but gently and the contents were carefully transferred to a sample bottle, narcotized with Alka-Seltzer, and preserved with the addition of sugar formaldehyde to form a 2% solution (Haney & Hall, 1973).

Preparation for counting and identifying zooplankton required measurement of the sample volume, gentle mixing of the sample, and removal of an aliquot with a Hensen-Stempel pipette. The aliquot was held in a 100 µm meshed bottom cup and rinsed with tap water to remove the preservative and finally it was poured into a circular counting dish. A minimum 550 zooplankton were identified for each sample. To count large predatory cladocerans, such as Bythotrephes, the whole sample was rinsed through a 600 µm mesh sieve, and all individuals were counted.

Taxonomic groups were identified and categorized in the following manner. For copepods, both cyclopoid and calanoid nauplii were combined, copepodites were identified to genus and adult copepods were identified to species. The cladocerans, both herbivorous and predatory, were identified to species.

To determine zooplankton biomasses, length measurements were made on a subsample of taxa (10 adult copepods and 25 copepodites or cladocerans) that were over 10% of the total density (using Image Pro Plus, image analysis software (Media Cybernetics, Silver Spring, MD). In the case of large predatory cladocerans, all individuals were measured or up to 100 individuals if more than that were present. Biomasses, expressed as dry weight, were determined using published length-weight regressions (Culver et al. 1985, Makarewicz and Jones 1990). For zooplankton taxa that comprised less than 10% of the total density, a default weight from the literature was used to determine biomass for all taxa except Bythotrephes (Hawkins and Evans 1979).
Availability date:
Metadata version: 3
Keydate: 2022-07-25 13:00:28+00
Editdate: 2024-09-10 14:32:20+00