# Blake Bahama Outer Ridge and Humber Estuary, UK Oxidizing Pre-Treatments on Amino Acids Data over the last 900ka #----------------------------------------------------------------------- # World Data Service for Paleoclimatology, Boulder # and # NOAA Paleoclimatology Program #----------------------------------------------------------------------- # Template Version 4.0 # Encoding: UTF-8 # NOTE: Please cite original publication, NOAA Landing Page URL, dataset and publication DOIs (where available), and date accessed when using downloaded data. # If there is no publication information, please cite investigator, study title, NOAA Landing Page URL, and date accessed. # # Description/Documentation lines begin with '#' followed by a space # Data lines have no '#' # # NOAA_Landing_Page: https://www.ncei.noaa.gov/access/paleo-search/study/38379 # Landing_Page_Description: NOAA Landing Page of this file's parent study, which includes all study metadata. # # Study_Level_JSON_Metadata: https://www.ncei.noaa.gov/pub/data/metadata/published/paleo/json/noaa-ocean-38379.json # Study_Level_JSON_Description: JSON metadata of this data file's parent study, which includes all study metadata. # # Data_Type: Paleoceanography # # Dataset_DOI: # # Science_Keywords: #--------------------------------------- # Resource_Links # # Data_Download_Resource: https://www.ncei.noaa.gov/pub/data/paleo/aar/millman2022/millman2022-1062b.txt # Data_Download_Description: NOAA Template File; 1062B, 1H-1 Amino Acids Data # #--------------------------------------- # Contribution_Date # Date: 2023-08-01 #--------------------------------------- # File_Last_Modified_Date # Date: 2023-08-01 #--------------------------------------- # Title # Study_Name: Blake Bahama Outer Ridge and Humber Estuary, UK Oxidizing Pre-Treatments on Amino Acids Data over the last 900ka #--------------------------------------- # Investigators # Investigators: Millman, Emily; Wheeler, Lucy; Billups, Katharina; Kaufman, Darrell; Penkman, Kirsty E.H. #--------------------------------------- # Description_Notes_and_Keywords # Description: #--------------------------------------- # Publication # Authors: Millman, E., Wheeler, L., Billups, K., Kaufman, D., Penkman, K.E.H. # Published_Date_or_Year: 2022 # Published_Title: Testing the effect of oxidizing pre-treatments on amino acids in benthic and planktic foraminifera tests # Journal_Name: Quaternary Geochronology # Volume: 73 # Edition: # Issue: # Pages: # Report_Number: # DOI: https://doi.org/10.1016/j.quageo.2022.101401 # Online_Resource: # Full_Citation: # Abstract: Amino acid racemization (AAR) is a geochronological method that uses the ratio of D- to L- configurations in optically active amino acids from carbonate fossils to determine the time elapsed since the death of an organism. Although AAR techniques have been widely applied to foraminiferal tests, there have been limited dedicated assessments of the potential of isolating a bleach-resistant, intra-crystalline fraction of proteins to improve the reliability of AAR in this biomineral system. In this study, we evaluate the effect of two oxidative pre-treatments (hydrogen peroxide and bleach) on amino acid concentrations and D/L values in sub-modern benthic foraminifers (Ammonia spp. and Haynesina germanica) and well-preserved mid Holocene and mid Pleistocene planktic foraminifers (Pulleniatina obliquiloculata, Globorotalia truncatulinoides, and Globorotalia tumida). The oxidative pre-treatments successfully reduced the amino acid content of the foraminiferal tests to a residual fraction, and with the exception of Ammonia spp., neither pre-treatment substantially affected the relative proportion of individual amino acids. The bleaching pre-treatment does not consistently alter D/L values when compared to peroxide pre-treatment, but it does tend to reduce the subsample variability in D/L values, albeit only to a small degree in an inconsistent fashion. Therefore, we recommend that a relatively weak oxidative pre-treatment with 3% hydrogen peroxide is sufficient for foraminifera-based AAR applications. #--------------------------------------- # Funding_Agency # Funding_Agency_Name: Petroleum Research Fund # Grant: PRF #56964-ND #--------------------------------------- # Funding_Agency # Funding_Agency_Name: University of York Chemistry Department # Grant: #--------------------------------------- # Funding_Agency # Funding_Agency_Name: National Science Foundation # Grant: #1855381 #--------------------------------------- # Funding_Agency # Funding_Agency_Name: European Research Council # Grant: #865222 #--------------------------------------- # Site_Information # Site_Name: ODP 1062B # Location: North Atlantic Ocean # Northernmost_Latitude: 28.246483 # Southernmost_Latitude: 28.246483 # Easternmost_Longitude: -74.407 # Westernmost_Longitude: -74.407 # Elevation_m: -4763 #--------------------------------------- # Data_Collection # Collection_Name: 1062B, 1H-1 Amino Acids Millman2022 # First_Year: 905400 # Last_Year: 0 # Time_Unit: calendar year before present # Core_Length_m: 239 # Parameter_Keywords: geochemistry # Notes: #--------------------------------------- # Chronology_Information # Chronology: #--------------------------------------- # Variables # PaST_Thesaurus_Download_Resource: https://www.ncei.noaa.gov/access/paleo-search/skos/past-thesaurus.rdf # PaST_Thesaurus_Download_Description: Paleoenvironmental Standard Terms (PaST) Thesaurus terms, definitions, and relationships in SKOS format. # # Data variables follow that are preceded by "##" in columns one and two. # Variables list, one per line, shortname-tab-var components: what, material, error, units, seasonality, data type, detail, method, C or N for Character or Numeric data) # ## Labcode sample identification,,,,,paleoceanography,,,C, ## Site sample identification,,,,,paleoceanography,,,N, ## Hole sample identification,,,,,paleoceanography,,,C, ## Core sample identification,,,,,paleoceanography,,,C, ## Section sample identification,,,,,paleoceanography,,,N, ## Top depth at sample end,,,centimeter,,paleoceanography,,,N,depth at top of core section ## Bottom depth at sample start,,,centimeter,,paleoceanography,,,N,depth at bottom of core section ## mcd depth,,,meter,,paleoceanography,,,N,mcd at the top of the core section ## Age age,,,calendar kiloyear before present,,paleoceanography,,,N,ages determined from %CaCO3 orbitally tuned data from Grützner et al. 2002 ## Species notes,,,,,paleoceanography,,,C,planktic foraminifera species used for measurements ## Pre-treatment notes,,,,,paleoceanography,,,C,pre-treatment method used prior to analysis; H2O2 is hydrogen peroxide treatment and NaOCl is bleach treatment ## Tests number of samples,,,count,,paleoceanography,,,N,number of foraminifera tests analyzed per sample ## Asp_D/L aspartic acid,foraminifer,,fraction,,paleoceanography,,high-performance liquid chromatography,N,ratio of D- to L-aspartic acid; reversed phase high performance liquid chromatography ## Glu_D/L glutamic acid,foraminifer,,fraction,,paleoceanography,,high-performance liquid chromatography,N,ratio of D- to L-glutamic acid; reversed phase high performance liquid chromatography ## L-Ser/L-Asp amino acid,foraminifer,,fraction,,paleoceanography,,high-performance liquid chromatography,N,ratio of L-serine to L-aspartic acid; reversed phase high performance liquid chromatography ## [Asp] aspartic acid,foraminifer,,picomole,,paleoceanography,,high-performance liquid chromatography,N,concentration of aspartic acid; reversed phase high performance liquid chromatography ## [Glu] glutamic acid,foraminifer,,picomole,,paleoceanography,,high-performance liquid chromatography,N,concentration of glutamic acid; reversed phase high performance liquid chromatography ## [Ser] serine,foraminifer,,picomole,,paleoceanography,,high-performance liquid chromatography,N,concentration of serine; reversed phase high performance liquid chromatography ## [Gly] glycine,foraminifer,,picomole,,paleoceanography,,high-performance liquid chromatography,N,concentration of glycine; reversed phase high performance liquid chromatography ## [Ala] alanine,foraminifer,,picomole,,paleoceanography,,high-performance liquid chromatography,N,concentration of alanine; reversed phase high performance liquid chromatography ## [Val] valine,foraminifer,,picomole,,paleoceanography,,high-performance liquid chromatography,N,concentration of valine; reversed phase high performance liquid chromatography ## [Phe] phenylalanine,foraminifer,,picomole,,paleoceanography,,high-performance liquid chromatography,N,concentration of phenylalanine; reversed phase high performance liquid chromatography ## [Ile] isoleucine,foraminifer,,picomole,,paleoceanography,,high-performance liquid chromatography,N,concentration of isoleucine; reversed phase high performance liquid chromatography ## [Leu] leucine,foraminifer,,picomole,,paleoceanography,,high-performance liquid chromatography,N,concentration of lecuine; reversed phase high performance liquid chromatography ## [Total] amino acid,foraminifer,,picomole,,paleoceanography,,high-performance liquid chromatography,N,total amino acid concentration; reversed phase high performance liquid chromatography #------------------------ # Data: # Data lines follow (have no #) # Data line format - tab-delimited text, variable short name as header # Missing_Values: NaN Labcode Site Hole Core Section Top Bottom mcd Age Species Pre-treatment Tests Asp_D/L Glu_D/L L-Ser/L-Asp [Asp] [Glu] [Ser] [Gly] [Ala] [Val] [Phe] [Ile] [Leu] [Total] 16611A 1062 B 1H 1 62 64 0.62 7.06 Globorotalia truncatulinoides H2O2 5 0.138 0.054 0.4 108 61 48 51 97 30 25 16 22 459 16611C 1062 B 1H 1 62 64 0.62 7.06 Globorotalia truncatulinoides H2O2 5 0.152 0.059 0.4 86 50 36 31 73 31 14 18 19 358 16611D 1062 B 1H 1 62 64 0.62 7.06 Globorotalia truncatulinoides H2O2 5 0.166 0.057 0.3 108 58 42 52 90 36 24 21 22 452 16611F 1062 B 1H 1 62 64 0.62 7.06 Globorotalia truncatulinoides H2O2 5 0.167 0.061 0.4 100 59 43 61 92 34 24 19 23 454 16611G 1062 B 1H 1 62 64 0.62 7.06 Globorotalia truncatulinoides H2O2 5 0.174 0.061 0.5 120 78 65 97 134 48 32 30 35 640 16611H 1062 B 1H 1 62 64 0.62 7.06 Globorotalia truncatulinoides H2O2 5 0.146 0.059 0.4 127 79 58 67 120 49 34 29 32 596 16611I 1062 B 1H 1 62 64 0.62 7.06 Globorotalia truncatulinoides H2O2 4 0.151 0.060 0.4 147 87 69 97 135 51 37 31 35 690 16611J 1062 B 1H 1 62 64 0.62 7.06 Globorotalia truncatulinoides H2O2 5 0.139 0.053 0.4 84 50 38 48 86 33 17 19 22 397 16611K 1062 B 1H 1 62 64 0.62 7.06 Globorotalia truncatulinoides NaOCl 10 0.124 0.053 0.5 18 25 9 19 45 21 2 10 6 155 16611L 1062 B 1H 1 62 64 0.62 7.06 Globorotalia truncatulinoides NaOCl 10 0.129 0.055 0.5 30 25 14 20 27 14 3 9 9 150 16611M 1062 B 1H 1 62 64 0.62 7.06 Globorotalia truncatulinoides NaOCl 10 0.134 0.059 0.4 33 29 13 23 45 20 3 13 11 190 16611T 1062 B 1H 1 62 64 0.62 7.06 Globorotalia truncatulinoides NaOCl 11 0.146 0.062 0.3 63 61 22 43 93 39 3 24 24 372