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HPLC_WB

Data Object HPLC pigments WB 
Contacts:
name        title
Dr Dennis McGillicuddy         Principal Investigator

Dataset description

Pigments from HPLC analysis of bottle samples collected during EDDIES Weatherbird cruises

 dates:           2004 - 2005
 location:        Sargasso Sea 
 project/cruise:  EDDIES/WB0409 2004 Transect 1 (EDT1)
                  EDDIES/WB0413 2004 Transect 2 (EDT2)
                  EDDIES/WB0506 2005 Transect 1 (EDT3)
                  EDDIES/WB0508 2005 Transect 2 (EDT4)
 platform:        R/V Weatherbird II


 Change history: YYMMDD
    070308: downloaded original data from EDDIES data Web site 
            (200609 DM FINAL_corrected.xls);
            prepared for OCB database by Nancy Copley, OCB DMO
    070313: added to OCB database by Cyndy Chandler, OCB DMO
            event number is from cruise event log
    070405: per Jason Perl, units for all pigment concentrations  
            changed from microgram/kilogram to microgram/liter 
 
 PI note: 080317: Recalibrated data are expected from NASA during the second half of 2008. 

Acquisition description

Methodology, protocols and PI-notes: NASA/TM-2003- HPLC Protocols (1 MB PDF)
J.L. Mueller, G.S. Fargion, C.R. McClain, C.C. Trees and J. Perl. 2003. "Ocean Optics Protocols For Satellite Ocean Color Sensor Validation, Revision 5, Volume V: Biogeochemical and Bio-Optical Measurements and Data Analysis Protocols". NASA Tech. Memo., NASA Goddard Space Flight Center, Greenbelt, Maryland. 43 pp. (PDF downlaoded from http://oceancolor.gsfc.nasa.gov/DOCS/)

Technician: Jason Perl (jperl@chors.sdsu.edu)
Center for Hydro-Optics & Remote Sensing
San Diego State University Research Foundation

Technician notes:
note 1: Did not have a signal for Chl_a @ 450nm (sample too dilute to get a chromatogram peak)
note 2: Did not have a signal for Chl_a @ 450nm or 436nm (sample too dilute to get a chromatogram peak)
note 3: no ancillary data; default volume filter set to 4000mL

chl_a_fluor and pheo_fluor are values from a Turner Fluorometer. Each HPLC extract is run on the Fluorometer as a second measure of Chla. Chla, MVa, DVa using the C18 HPLC method, yield a single peak for Chla, measured at 436nm and 450nm. A regression equation, between the 2 wavelengths (based on calibration curves of 100% MVa and 100% DVa), is used to give values for each pigment within a single peak. Chla @436nm is reported for comparison with legacy data (that are often only listed simply Chla).

Processing description

DMO notes:
Original Excel file downloaded from EDDIES Web site: copy of original Excel file

The comments in the data object were taken from the 061127 version of file readme_jason_notes.txt.
SID_Filter and SID_PI were identical but some samples were also included another sample designation that was a consecutive SID_PI. In these cases, the consecutive SID replaced the SID_PI.


Field Names List

ParameterDescriptionUnits
Cruise_IDcruise ID designation codealphanumeric
eventunique sampling event numberYYYYMMDDhhmm
datedate (GMT); start of samplingYYYYMMDD
timetime of measurement (GMT)hhmm
lonlongitude at start of measurement; negative denotes Westdecimal degrees
latlatitude at start of measurement; negative denotes Southdecimal degrees
stastation numberdimensionless
depth_ndepth; nominalmeters
run_ID_HPLCsample run ID given by HPLC labalphanumeric
run_PIsample run ID given by PIalphanumeric
run_filt_IDsample run filter IDalphanumeric
vol_filt_mlvolume water filtered for analysismilliliters
chl_a_fluorchlorophyll-a by Fluorometric methodsmicrogram/liter
pheo_fluorpheopigments measured by Fluorometric methodsmicrogram/liter
chlide_aHPLC Chlorophyllide amicrogram/liter
phideHPLC Pheophorbide amicrogram/liter
pheoHPLC Pheophytin amicrogram/liter
chl_a_allomerHPLC chlorophyll a allomermicrogram/liter
chl_aHPLC chlorophyll a (measured at 436nm)microgram/liter
chl_a_epimerHPLC chlorophyll a epimermicrogram/liter
chl_c3HPLC Chlorophyll_c3microgram/liter
chl_c2HPLC Chlorophyll_c2microgram/liter
peridininHPLC Peridininmicrogram/liter
fucox_butHPLC 19' - Butanoyloxyfucoxanthinmicrogram/liter
fucoxHPLC fucoxanthinmicrogram/liter
fucox_hexHPLC 19' - Hexanoyloxyfucoxanthinmicrogram/liter
neoxHPLC neoxanthinmicrogram/liter
prasinoxHPLC Prasinoxanthinmicrogram/liter
violaxHPLC violaxanthinmicrogram/liter
diadinoxHPLC Diadinoxanthinmicrogram/liter
alloxHPLC Alloxanthinmicrogram/liter
diatoxHPLC Diatoxanthinmicrogram/liter
luteinHPLC Luteinmicrogram/liter
zeaxHPLC Zeaxanthinmicrogram/liter
chl_bHPLC Chlorophyll bmicrogram/liter
caroteneHPLC Total Carotenes (alpha + beta)microgram/liter
chl_a_mvMonovinyl chlorophyll a; based on the C18 method regression between 436nm and 450nmmicrogram/liter
chl_a_dvDivinyl chlorophyll a; based on the C18 method regression between 436nm and 450nmmicrogram/liter
chl_a_totTotal Chlorophyll a =chlide_a +allomer +epimer +MVa +Dvamicrogram/liter
commentscomments and notes text

Platforms List

R/V Weatherbird II WB0409 R/V Weatherbird II WB0413 R/V Weatherbird II WB0506 R/V Weatherbird II WB0508

Instruments List

  1. Niskin Bottle:


This document is created from the content of the BCO-DMO metadata database.    2010-11-17  09:39:34

info app: /home/ocb/dbase-v2 v.090827 CLC
Data URL: http://ocb.whoi.edu/jg/serv/OCB/EDDIES/multi-ship/HPLC_WB.