Contacts:Data Object HPLC pigments WB
name | title |
---|---|
Dr Dennis McGillicuddy | Principal Investigator |
dates: 2004 - 2005 location: Sargasso Sea project/cruise: EDDIES/WB0409 2004 Transect 1 (EDT1) EDDIES/WB0413 2004 Transect 2 (EDT2) EDDIES/WB0506 2005 Transect 1 (EDT3) EDDIES/WB0508 2005 Transect 2 (EDT4) platform: R/V Weatherbird II Change history: YYMMDD 070308: downloaded original data from EDDIES data Web site (200609 DM FINAL_corrected.xls); prepared for OCB database by Nancy Copley, OCB DMO 070313: added to OCB database by Cyndy Chandler, OCB DMO event number is from cruise event log 070405: per Jason Perl, units for all pigment concentrations changed from microgram/kilogram to microgram/liter PI note: 080317: Recalibrated data are expected from NASA during the second half of 2008.
Methodology, protocols and PI-notes: NASA/TM-2003- HPLC Protocols (1 MB PDF)
J.L. Mueller, G.S. Fargion, C.R. McClain, C.C. Trees and J. Perl. 2003.
"Ocean Optics Protocols For Satellite Ocean Color Sensor Validation,
Revision 5, Volume V: Biogeochemical and Bio-Optical Measurements
and Data Analysis Protocols". NASA Tech. Memo., NASA Goddard Space
Flight Center, Greenbelt, Maryland. 43 pp.
(PDF downlaoded from http://oceancolor.gsfc.nasa.gov/DOCS/)
Technician: Jason Perl (jperl@chors.sdsu.edu)
Center for Hydro-Optics & Remote Sensing
San Diego State University Research Foundation
Technician notes:
note 1: Did not have a signal for Chl_a @ 450nm
(sample too dilute to get a chromatogram peak)
note 2: Did not have a signal for Chl_a @ 450nm or 436nm
(sample too dilute to get a chromatogram peak)
note 3: no ancillary data; default volume filter set to 4000mL
chl_a_fluor and pheo_fluor are values from a Turner Fluorometer. Each HPLC extract is run on the Fluorometer as a second measure of Chla. Chla, MVa, DVa using the C18 HPLC method, yield a single peak for Chla, measured at 436nm and 450nm. A regression equation, between the 2 wavelengths (based on calibration curves of 100% MVa and 100% DVa), is used to give values for each pigment within a single peak. Chla @436nm is reported for comparison with legacy data (that are often only listed simply Chla).
DMO notes:
Original Excel file downloaded from EDDIES Web site:
copy of original Excel file
The comments in the data object were taken from the 061127 version of file
readme_jason_notes.txt.
SID_Filter and SID_PI were identical but some samples
were also included another sample designation that was a consecutive SID_PI.
In these cases, the consecutive SID replaced the SID_PI.
Parameter | Description | Units |
---|---|---|
Cruise_ID | cruise ID designation code | alphanumeric |
event | unique sampling event number | YYYYMMDDhhmm |
date | date (GMT); start of sampling | YYYYMMDD |
time | time of measurement (GMT) | hhmm |
lon | longitude at start of measurement; negative denotes West | decimal degrees |
lat | latitude at start of measurement; negative denotes South | decimal degrees |
sta | station number | dimensionless |
depth_n | depth; nominal | meters |
run_ID_HPLC | sample run ID given by HPLC lab | alphanumeric |
run_PI | sample run ID given by PI | alphanumeric |
run_filt_ID | sample run filter ID | alphanumeric |
vol_filt_ml | volume water filtered for analysis | milliliters |
chl_a_fluor | chlorophyll-a by Fluorometric methods | microgram/liter |
pheo_fluor | pheopigments measured by Fluorometric methods | microgram/liter |
chlide_a | HPLC Chlorophyllide a | microgram/liter |
phide | HPLC Pheophorbide a | microgram/liter |
pheo | HPLC Pheophytin a | microgram/liter |
chl_a_allomer | HPLC chlorophyll a allomer | microgram/liter |
chl_a | HPLC chlorophyll a (measured at 436nm) | microgram/liter |
chl_a_epimer | HPLC chlorophyll a epimer | microgram/liter |
chl_c3 | HPLC Chlorophyll_c3 | microgram/liter |
chl_c2 | HPLC Chlorophyll_c2 | microgram/liter |
peridinin | HPLC Peridinin | microgram/liter |
fucox_but | HPLC 19' - Butanoyloxyfucoxanthin | microgram/liter |
fucox | HPLC fucoxanthin | microgram/liter |
fucox_hex | HPLC 19' - Hexanoyloxyfucoxanthin | microgram/liter |
neox | HPLC neoxanthin | microgram/liter |
prasinox | HPLC Prasinoxanthin | microgram/liter |
violax | HPLC violaxanthin | microgram/liter |
diadinox | HPLC Diadinoxanthin | microgram/liter |
allox | HPLC Alloxanthin | microgram/liter |
diatox | HPLC Diatoxanthin | microgram/liter |
lutein | HPLC Lutein | microgram/liter |
zeax | HPLC Zeaxanthin | microgram/liter |
chl_b | HPLC Chlorophyll b | microgram/liter |
carotene | HPLC Total Carotenes (alpha + beta) | microgram/liter |
chl_a_mv | Monovinyl chlorophyll a; based on the C18 method regression between 436nm and 450nm | microgram/liter |
chl_a_dv | Divinyl chlorophyll a; based on the C18 method regression between 436nm and 450nm | microgram/liter |
chl_a_tot | Total Chlorophyll a =chlide_a +allomer +epimer +MVa +Dva | microgram/liter |
comments | comments and notes | text |
R/V Weatherbird II WB0409 | R/V Weatherbird II WB0413 | R/V Weatherbird II WB0506 | R/V Weatherbird II WB0508 |
This document is created from the content of the BCO-DMO metadata database. 2010-11-17 09:39:34
info app: /home/ocb/dbase-v2 v.090827 CLC