ECOHAB: Mote Process Cruises 1998 through 2000
Identification_Information
Data_Quality_Information
Entity_and_Attribute_Information
Distribution_Information
Metadata_Reference_Information
Identification Information
Section Index
Citation:
Citation Information:
Title: ECOHAB: Mote Process Cruises 1998 through 2000
Description:
Abstract:
Harmful algal blooms of the toxic dinoflagellate, Karenia brevis, have caused massive fish kills in the Gulf of Mexico since the 1500's, with most occurrences on the west coast of Florida. In 1996, the list of states that have experiences natural resource, public health and economic impacts related to this organism expanded, with the addition of Alabama, Mississippi, and Louisiana, to include all the Gulf-coast states and North Carolina. Estimates of economic impacts to Florida and North Carolina from two moderate intensity blooms ranged from 15 to 25 million dollars respectively. The harmful impacts caused by K. brevis occur only when cell concentrations increase significantly above low background concentrations that are present year-round in the Eastern Gulf of Mexico. Once a bloom has developed offshore in typically oligotrophic waters, cell concentrations at the 105 level can be maintained for months. During 21 of the past 22 years, red tide blooms have been observed within the region between Tampa Bay and Charlotte Harbor.
The key to understanding any HAB lies in knowing how one algal species has adapted and come to dominate in its particular realm of physical, biological and chemical conditions. Our ability to predict initiation, maintenance, and dispersal of blooms on the Florida shelf has been severely limited by the lack of a quantitative description, or model, of their population dynamics and the physical, biological and chemical regime in which they are embedded. The modeling components of this project will incorporate the quantitative description of blooms and their surrounding environment provided by the field and laboratory portions of this project. The field component will employ a set of annual process cruises.
Purpose:
1. Model the initiation, maintenance, and export of K. brevis red tides on the west Florida shelf at different time
and space scales to predict landfall
2. Describe the physical habitat that effects transport and concentration of K. brevis
3. Determine the sources of inorganic and organic nutrients that allow growth and persistence of large K. brevis
populations in coastal waters
4. Determine the interactions of cellular, behavioral, life cycle, and community regulation processes with environmental forcing factors during stages of bloom development.
5. Determine the production, occurrence, fate, and effects of brevetoxins in the environment during and after K. brevis blooms.
Supplemental Information:
ECOHAB (ECology and Oceanography of Harmful Algal Blooms) is a scientific program designed to increase understanding of the fundamental processes underlying the impacts and population dynamics of HABs. This program addresses the many factors at organismal, population, community, and ecosystem levels that determine how HAB species respond to, and potentially alter their environment, the manner in which HAB species affect food-web and community interactions. The program is based on the results of a workshop co-sponsored by National Oceanic and Atmospheric Administration (NOAA) and National Science Foundation (NSF) and currently receives funding from several federal partners including NOAA, NSF, the U.S. Navy and the U.S. Environmental Protection Agency (EPA).
Time Period of Content:
Time Period Information:
Range of Dates/Times:
Beginning Date: 9/1998
Ending Date: 9/2001
Currentness Reference: Observed
Status:
Progress: In work
Maintenance and Update Frequency: Annually
Spatial Domain:
Description of Geographic Extent: Eastern Gulf of Mexico (site-specific for locations of K.brevis blooms).
Bounding Coordinates:
West Bounding Coordinate: -87.23565
East Bounding Coordinate: -81.71588
North Bounding Coordinate: 30.39237
South Bounding Coordinate: 25.44867
Keywords:
Theme:
Theme Keyword Thesaurus: None
Theme Keyword: algae
Theme Keyword: aquatic
Theme Keyword: botany
Theme Keyword: chemistry
Theme Keyword: distribution
Theme Keyword: dynamic
Theme Keyword: marine
Theme Keyword: natural resource
Theme Keyword: nitrate
Theme Keyword: nutrient
Theme Keyword: organic
Theme Keyword: phosphorus
Theme Keyword: physical oceanography
Theme Keyword: physical processes
Theme Keyword: plankton
Theme Keyword: seasonally
Theme Keyword: succession
Theme Keyword: water chemistry
Theme Keyword: water quality
Theme Keyword: brevetoxin
Theme Keyword: pigments
Theme Keyword: aerosol
Place:
Place Keyword Thesaurus: None
Place Keyword: Florida
Place Keyword: Gulf of Mexico
Place Keyword: West Florida Shelf
Place Keyword: Florida panhandle
Stratum:
Stratum Keyword Thesaurus: None
Stratum Keyword: surface
Stratum Keyword: benthic sampling
Stratum Keyword: profiles
Temporal:
Temporal Keyword Thesaurus: None
Temporal Keyword: annual
Temporal Keyword: daily measurements of one location
Taxonomy:
Keywords/Taxon:
Taxonomic Keyword Thesaurus: None
Taxonomic Keywords: Ptychodiscus brevis
Taxonomic Keywords: Gymnodinium breve
Taxonomic Keywords: Karenia brevis
Taxonomic System:
Classification System/Authority:
Classification System Citation:
Citation:
Citation Information:
Title: Tomas, C. R. (Ed.) 1997. Identifying Marine Phytoplankton. Academic Press, 558 pp.
Identification Reference:
Citation Information:
Originator: Charles C. Davis
Publication Date: March 1948
Title: Gymnodinium brevis sp. Nov., A Cause of Discolored Water and Animal Mortality in the Gulf of Mexico
Edition: The Botanical Gazette Vol. 109, No. 3
Series Information:
Series Name: The Botanical Gazette
Issue Identification: Vol. 109, No. 3
Publication Information:
Identification Reference:
Citation Information:
Originator: M-J. Chretiennot-Dinet, A. Sournia, M. Ricard and C. Billlard
Publication Date: 1993
Title: A classification of the marine phytoplankton of the world from class to genus
Series Information:
Series Name: Phycologia
Issue Identification: Volume 32 (3)
Taxonomic Procedures: Sampled water
Taxonomic Classification:
Taxon Rank Name: Division- Phyrrophyta
Taxonomic Classification:
Taxon Rank Name: Class Dinophyceae
Taxonomic Classification:
Taxon Rank Name: Order- Gymnodiniales
Taxonomic Classification:
Taxon Rank Name: Family- Gymnodiniaceae
Taxonomic Classification:
Taxon Rank Name: Genus- Karenia
Applicable Common Name: Ptychodiscus
Applicable Common Name: Gymnodinium
Taxonomic Classification:
Taxon Rank Name: species- brevis
Applicable Common Name: breve
Access Constraints:
Data will be made available to other investigators and the public after the completion of ECOHAB: Florida,
projected to be 2002, in a synthesized format.
Use Constraints:
Principal investigator of the study retains intellectual rights to synthesize data for publication and distribution. If a future user obtains permission to use any of the data, then that user should acknowledge the source.
Point of Contact:
Contact Information:
Contact Person Primary:
Contact Person: Dr. Gary Kirkpatrick
Contact Organization: Mote Marine Laboratory
Contact Position: Senior Scientist
Contact Address:
Address Type: mailing and physical address
Address: 1600 Ken Thompson Parkway
City: Sarasota
State or Province: FL
Postal Code: 34236
Country: US
Contact Voice Telephone: (941) 388-4441
Contact Facsimile Telephone: (941) 388-4312
Contact Electronic Mail Address: gkirkpat@mote.org
Browse Graphic:
Browse Graphic:
Data Set Credit: Brad Pederson, Gary Kirkpatrick, Mike Henry, Richard Pierce, and Kelly Dixon of Mote Marine Laboratory
Native Data Set Environment:
Format- Surfer, Microsoft Excel, Microsoft Access
Type/File Extension- .srf, .xls, .dbf
Form- table, spreadsheet, maps, database
Media Form- digital
Computer Hardware- PC
Operating System Used- Windows 95, 98, 2000
Cross Reference:
Citation Information:
Title: ECOHAB: Kamykowski_D- Florida Process Cruise 11/16-19/1998- Karenia brevis counts, biochemistry and behavior
Cross Reference:
Citation Information:
Originator: Mark Geesey and Mary Culver
Title: ECOHAB: Culver_M- NOAA CSC/Coastal Remote Sensing Cruise APR99FLA: West Florida Coast Cruise, April 1999
Other Citation Details: APR99FLA Cruise Report
Online Linkage: http://www.csc.noaa.gov/crs/cruises/apr99fla/index.html
Cross Reference:
Citation Information:
Title: ECOHAB: Kamykowski_D- Florida Process Cruise 09/23-30/1999 - Karenia brevis counts, biochemistry and nitrate.
Cross Reference:
Citation Information:
Title: ECOHAB: Landsburg_J- Fate and Effects of Breve Toxin
Cross Reference:
Citation Information:
Title: ECOHAB: Steidinger_K- Karenia brevis Life Cycle Studies
Cross Reference:
Citation Information:
Title: ECOHAB: Van Dolah_F- Karenia brevis Cell Cycle Analysis for Determination of In Situ Growth Rates
Cross Reference:
Citation Information:
Title: ECOHAB: Millie_D-Pelican Process Cruise HPLC Pigment analysis 2000
Cross Reference:
Citation Information:
Title: ECOHAB: Mote Monthly transect cruises
Cross Reference:
Citation Information:
Title: ECOHAB: Redalje_D-Nutrient and Growth Dynamics of Karenia brevis in the Gulf of Mexico
Cross Reference:
Citation Information:
Title: ECOHAB: Lohrenz_S- Subcellular carbon assimilation patterns and optical characterization of Karenia brevis populations
Cross Reference:
Citation Information:
Originator: Ajit Subramaniam, REMSA, NOAA Coastal Services Center,Mary Culver, NOAA Coastal Services Center,Mark Geesey, REMSA, NOAA Coastal Services Center
Publication Date: 200009
Title: ECOHAB: Culver_M- NOAA CSC/CRS Cruise APR00FWS: West Florida Shelf Cruise, April 2000
Geospatial Data Presentation Form: profile
Series Information:
Series Name: CSC Technical Report
Issue Identification: NOAA/CSC/20018-PUB
Publication Information:
Publication Place: Charleston, South Carolina
Publisher: NOAA Coastal Services Center
Online Linkage: http://www.csc.noaa.gov/crs/cruises/apr00fws/index.html
Data Quality Information
Section Index
Attribute Accuracy:
Attribute Accuracy Report: Within limits of differential GPS accuracy
Positional Accuracy:
Vertical Positional Accuracy:
Vertical Positional Accuracy Report: plus/minus 1-2 meters
Lineage:
Methodology:
Methodology Type: Field
Methodology Description:
Assumptions associated with experimental design:
That a red-tide bloom would occur within the region being surveyed on an annual basis.
Sampling Information:
Sampling Types: water, algae
Sampling Strategy: Bloom patches
Data Collection Type: field collection
On What Basis Were the Sampling Locations Chosen?
Sampling location is based on bloom position.
Sampling Sites are numerous. See data for lat. longs.
Sampling Frequency: Annual
Replicates Were Taken Sometimes
Field Collection Techniques used: CTD, 2.5 and 5 l Niskin bottle
For Samples Taken Back to the Lab:
Field Preservation Methods used:
K. brevis Cell Concentration: Counts performed immediately, no preservation required.
Total Dissolved Phosphorus: On ice
Dissolved NO2&3: On Ice
Dissolved NH4: On Ice
Total Persulfate N: On Ice
Total Persulfate P: On Ice
Dissolved Organic Nitrogen: On ice
Particulate Phosphorus: On ice
Particulate Nitrogen: on ice
Dissolved Persulfate N: On Ice
Dissolved persulfate P: On Ice
Dissolved SiO2: On Ice
Chlorophyll a: On ice
Storage Preservation Method:
K. brevis Cell Concentration: Lugol staining
Total Dissolved Phosphorus: Freezing
Dissolved NO2&3: Freezing
Dissolved NH4: Freezing
Total Persulfate N: Freezing
Total Persulfate P: Freezing
Dissolved Organic Nitrogen: Freezing
Particulate Phosphorus: Freezing
Particulate Nitrogen: Freezing
Dissolved Persulfate N: Freezing
Dissolved persulfate P: Freezing
Dissolved SiO2: Freezing
Chlorophyll a: Freezing
Typical Storage Time Before Analysis:
K. brevis Cell Concentration: no storage time- counts done immediately
Total Dissolved Phosphorus: 2 weeks
Dissolved NO2&3: 2 weeks
Dissolved NH4: 2 weeks
Total Persulfate N: 2 weeks
Total Persulfate P: 2 weeks
Dissolved Organic Nitrogen: 2 weeks
Particulate Phosphorus: 2 weeks
Particulate Nitrogen: 2 weeks
Dissolved Persulfate N: 2 weeks
Dissolved persulfate P: 2 weeks
Dissolved SiO2: 2 weeks
Chlorophyll a: 2 weeks
Special Processing Methods Prior to Lab Analysis:
K. brevis Cell Concentration: none
Total Dissolved Phosphorus: None
Dissolved NO2&3: None
Dissolved NH4: None
Total Persulfate N: None
Total Persulfate P: None
Dissolved Organic Nitrogen: None
Particulate Phosphorus:
Particulate Nitrogen:
Dissolved Persulfate N: None
Dissolved persulfate P: None
Dissolved SiO2: None
Chlorophyll a: None
Lab Analysis Methods Used:
K. brevis Cell Concentration: Microscopic Count
Total Dissolved Phosphorus: Standard Method (SM) 18 4500-P-F
Dissolved NO2&3: EPA1 353.2
Dissolved NH4: SM18 4500-NH3-H
Total Persulfate N: D’Elia, Streudler and Corwin. 1977. Limnol. & Oceanogr. 22: 760-764
Total Persulfate P: D’Elia, Streudler and Corwin. 1977. Limnol. & Oceanogr. 22: 760-764
Dissolved Organic Nitrogen:
Particulate Phosphorus:
Particulate Nitrogen:
Dissolved Persulfate N: D’Elia, Streudler and Corwin. 1977. Limnol. & Oceanogr. 22: 760-764
Dissolved persulfate P: D’Elia, Streudler and Corwin. 1977. Limnol. & Oceanogr. 22: 760-764
Dissolved SiO2: EPA1 370.1
Chlorophyll a: SM 10200 H2
Data Recording Methods Used: LIMS, manual
Data Reduction Techniques Employed: spreadsheet, graphs, maps
Data Reporting Methods: reports, meetings
Methodology:
Methodology Type: brevetoxin analysis in water
Methodology Description:
Total brevetoxins in sea water are established by extraction and recovery according to the method of using a C18 bonded-phase extraction disc (Metachem, Torrance, CA) for toxin recovery. 3.5-L of culture is filtered through a glass-fiber filter GF/D (Whatman, Clifton, NJ) ), the cells are lysed by the addition of distilled water and the free brevetoxins are collected in the filtrate. The entire sample is filtered through a C18 extraction disc to recover brevetoxins. The brevetoxins are eluted from the disc with 20 ml methanol under vacuum and reduced to 3 ml volume in preparation for analysis by high performance liquid chromatography (HPLC). Standard brevetoxins PbTx-2 and PbTx-3 are obtained from Chiral Corp. Miami, FL. Verification of PbTx-1 and PbTx-5 is accomplished by secondary standards recovered from K. brevis culture and identified by HPLC retention values.
The amounts of intra- and extra-cellular toxins are determined in a 5-L sample processed through a stirred ultra-filtration cell concentrator (Amicon, Beverly MA). The sample was filtered through a 0.8 micron polycarbonate membrane (Osmonics, Westborough, MA) in the stirred cell at 5 psi to gently collect the viable K. brevis cells (intra-cell toxins) on the filter and to allow extra-cellular toxins to pass through with the filtrate. The Extra-Cellular toxins are recovered by passing the 4.8 L of filtrate through a C18 bonded-phase extraction disc as above. The Intra-cell toxins are extracted from approximately 200ml of solution remaining above the membrane (containing viable K. brevis cells) by rinsing the cell and membrane with distilled water and recovering toxins on a C18 extraction disc as above. Methanol extracts are reduced in volume to 3 ml in preparation for HPLC analysis as above.
Aerosol samples are collected using a high volume air sampler (Tisch Environmental, Inc. Cleves, OH) fitted with a 8 inch by 10 inch glass fiber filter (Whatman). Samples are collected in 4 to9 hour intervals while steaming into the wind. The power is discontinued when the ship stopped on station during the aerosol collection interval. Power is resupplied when the ship continued on course. The volume of air is recorded using a water manometer vacuum gauge. Filters are removed and placed in precleaned glass jars with teflon lids. The filters are covered with acetone and stored in a freezer for future transport to the lab.
Filters are extracted using a soxhlet apparatus for a minimum of 12 hours in methanol. Extracts were concentrated to 3 ml and injected into a HPLC-uv (Varian 5000) with detection at 215 nm. The mobile phase was 85:15 methanol/water with a flow rate of 1 ml/min. The stationary phase was a 25 cm by 5 mm C-18 column (Burdick & Jackson, OD5). All data was reduced using a PE-Nelson Turbochrom workstation (Perkin Elmer, Norwalk, CT).
Sediment samples are colleted using a Van Veen sampler hoisted with an “A” frame from the stern of the ship. This device is selected to provide an undisturbed surface from which to sample. Samples are collected in precleaned glass jars with teflon lined lids by scraping the top cm of sediment. The jars are filled one third full and methanol was added to generously cover the sample. Sediments are extracted using a soxhlet apparatus for a minimum of 12 hours in methanol. Extracts are concentrated to dryness and transferred to dichloromethane for silica and alumina column clean up. Final fractions are evaporated to dryness and dissolved in methanol for HPLC-uv analysis as described above.
Methodology:
Methodology Type: HPLC Pigments
Methodology Description:
Lab Analysis Methods Used:
Photopigments: Photopigments are analyzed using high-performance liquid chromatography (HPLC). Aliquots of water were drawn under low vacuum onto GF/F glass-fiber filters that were immediately frozen and stored at -80 oC. At time of analysis, frozen filters were placed in 100% acetone, sonicated, and extracted in darkness at -20 oC for 12 to 16 hrs.
Filtered extracts (up to 200 Error! Bookmark not defined.l) were injected directly into either 1) a Hewlett Packard (HP) model 1090 HPLC equipped with a single monomeric (ODS Hypersil; 100 x 4.6mm, 5 m) and two polymeric (250 x 4.6, 5 :m) reverse-phase C18 columns in series, a photodiode array detector, and a HP Model 1046A fluorometer placed in-line or 2) a Shimadzu model LC-XXX HPLC equipped with an ODS-Hypersil C18 column (200 x 4.6 mm, 4m particle size) and a diode array detector. The mobile phases and solvent flow rates followed that described by Pinckney et al. (1996) –for the HP – or Wright et al. (1991) – for the Shimadzu. Pigments were identified and quantified using authentic standards for chlorophylls and carotenoids. Absolute and relative phylogenetic group (and potentially species-specific) contributions to total Chl a biomass are derived from chemotaxonomic photopigments using ChemTax® software (Mackey et al 1996).
Community Production: Bulk carbon fixation will be measured using 14C uptake experiments. Small volume samples (6-ml) inoculated with 14C are incubated in a temperature-controlled, variable-light photosynthetron (Prézelin et al. 1992) for 40 minutes. Samples are acidified in vials, transferred to the laboratory in darkness and and total activity later determined using a LKB model XXXX scintillation counter. From P-I measurements, non-linear fitting techniques will be used to determine the following P-I parameters; Pmax (maximum light saturated rate, a (light limited slope), Ik (minimum irradiance required to light-saturate photosynthesis) and b (slope at photoinhibiting irradiances) as described in Fahnenstiel et al. (1989).
Subsurface irradiance will be combined with P-I measurements in a bio-optical primary production model (e.g. Great Lakes Production Model; Lang & Fahnenstiel, 1996) allowing for estimates of phytoplankton production.
Entity and Attribute Information
Section Index
Detailed Description:
Entity Type:
Entity Type Label: Water Quality-
Entity Type Definition: Chemical parameters including nutrients, metals, dissolved oxygen, extracted chl, and fluorescence associated with red tide blooms.
Attribute:
Attribute Label: Bottle
Attribute Definition: CTD bottle number.
Attribute:
Attribute Label: CellCount
Attribute Definition: K. brevis cell count (integer)
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: cells/l
Attribute:
Attribute Label: Chl a
Attribute Definition: uncorrected chl a conc. (Number)
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U0.5
Range Domain Maximum: TBD
Attribute Units of Measure: ug/l
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Beginning Date of Attribute Values: x
Ending Date of Attribute Values: x
Attribute:
Attribute Label: Chl a Corrected
Attribute Definition: chlorophyll a conc corrected for phaeophytin (number)
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: U0.5
Range Domain Maximum: TBD
Attribute Units of Measure: ug/l
Beginning Date of Attribute Values: x
Ending Date of Attribute Values: x
Attribute:
Attribute Label: Chl b
Attribute Definition: chl b conc. (Number)
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U0.5
Range Domain Maximum: TBD
Attribute Units of Measure: ug/l
Ending Date of Attribute Values: x
Attribute:
Attribute Label: Chl c
Attribute Definition: chl c conc. (Number)
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: U0.5
Range Domain Maximum: TBD
Attribute Units of Measure: ug/l
Beginning Date of Attribute Values: x
Ending Date of Attribute Values: x
Attribute:
Attribute Label: Cpar
Attribute Definition: Corrected photosynthically available radiation (number).
Attribute Domain Values:
Range Domain:
Attribute Units of Measure: umol/m2/sec
Attribute:
Attribute Label: Date
Attribute Definition: Date sample is collected (integer)
Attribute:
Attribute Label: DepS
Attribute Definition: Seawater depth at which sample was taken (number).
Attribute Domain Values:
Range Domain:
Attribute Units of Measure: meter
Attribute:
Attribute Label: FlS
Attribute Definition: Relative fluorescence as measured by CTD (number).
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute:
Attribute Label: Latitude
Attribute Definition: position latitude (number)
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: 26
Range Domain Maximum: 28
Attribute Units of Measure: decimal degrees
Attribute:
Attribute Label: Longitude
Attribute Definition: position longitude (number)
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: -82
Range Domain Maximum: -84
Attribute Units of Measure: decimal degree
Attribute:
Attribute Label: NH4-N Dissolved
Attribute Definition: dissolved ammonia concentration (number)
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default
Range Domain:
Range Domain Minimum: U0.005
Range Domain Maximum: TBD
Attribute Units of Measure: mg/l
Beginning Date of Attribute Values: x
Ending Date of Attribute Values: x
Attribute:
Attribute Label: NO23-N Dissolved
Attribute Definition: dissolved nitrite and nitrate
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: U0.005
Range Domain Maximum: TBD
Attribute Units of Measure: mg/l
Beginning Date of Attribute Values: x
Ending Date of Attribute Values: x
Attribute:
Attribute Label: OxMg/L
Attribute Definition: Dissolved oxygen as measured by CTD (number)
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: 20
Attribute Units of Measure: mg/l
Attribute:
Attribute Label: Par
Attribute Definition: Raw photosynthically available radiation (number).
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: X
Attribute:
Attribute Label: Phaeophytin a
Attribute Definition: Phaeophytin a conc (number)
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U0.5
Range Domain Maximum: TBD
Attribute Units of Measure: ug/l
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Beginning Date of Attribute Values: x
Ending Date of Attribute Values: x
Attribute:
Attribute Label: PO4-P Dissolved
Attribute Definition: dissolved phosphate concentration (number)
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: U0.005
Range Domain Maximum: TBD
Attribute Units of Measure: mg/l
Beginning Date of Attribute Values: x
Ending Date of Attribute Values: x
Attribute:
Attribute Label: Pr
Attribute Definition: Water Pressure at depth sampled.
Attribute Domain Values:
Range Domain:
Attribute Units of Measure: X
Attribute:
Attribute Label: SiO2 Dissolved
Attribute Definition: dissolved silicate (number)
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: U0.05
Range Domain Maximum: TBA
Attribute Units of Measure: mg/l
Attribute:
Attribute Label: Spar
Attribute Definition: photosynthically available radiation at surface (on deck).
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: umol/m2/sec
Attribute:
Attribute Label: Station
Attribute Definition: Station identifier
Attribute Domain Values:
Range Domain:
Attribute Units of Measure: character
Attribute:
Attribute Label: Time
Attribute Definition: Time sample collected (integer)
Attribute Domain Values:
Range Domain:
Attribute Units of Measure: hh:mm:ss
Attribute:
Attribute Label: TimeJ
Attribute Definition: Time and date of sampling based on Julian time (365 day time scale)
Attribute Domain Values:
Range Domain:
Attribute Units of Measure: X
Attribute:
Attribute Label: Total persulfate N
Attribute Definition: total nitrogen (number)
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: U0.005
Range Domain Maximum: TBD
Attribute Units of Measure: mg/l
Beginning Date of Attribute Values: X
Ending Date of Attribute Values: X
Attribute:
Attribute Label: Total Persulfate N Dissolved
Attribute Definition: total nitrogen dissolved (number)
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U0.005
Range Domain Maximum: TBA
Attribute Units of Measure: mg/l
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Beginning Date of Attribute Values: X
Ending Date of Attribute Values: X
Attribute:
Attribute Label: Total Persulfate P
Attribute Definition: total phosphorus (number)
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U0.005
Range Domain Maximum: TBD
Attribute Units of Measure: mg/l
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: Default value
Beginning Date of Attribute Values: X
Ending Date of Attribute Values: x
Attribute:
Attribute Label: Total Persulfate P Dissolved
Attribute Definition: total phosphorus dissolved (number)
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U0.005
Range Domain Maximum: TBD
Attribute Units of Measure: mg/l
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Beginning Date of Attribute Values: x
Ending Date of Attribute Values: x
Attribute:
Attribute Label: Turbidity (CTD)
Attribute Definition: turbidity by CTD (number)
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: FTU
Beginning Date of Attribute Values: x
Ending Date of Attribute Values: x
Detailed Description:
Entity Type:
Entity Type Label: brevetoxin measurements
Entity Type Definition:
Breve toxin analysis total and/or dissolved and particulate (including intra-cellular) distribution in water, sediments and air.The number following the PbTx-identifier signifies the structure of the compound. There are several toxins associated with G. breve and this is the current nomenclature assigned to the different structures.
Attribute:
Attribute Label: PbTx-1
Attribute Definition: concentration of brevetoxin-1(number) . Detection limits are based on sea water samples.
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: U0.8
Range Domain Maximum: TBA
Attribute Units of Measure: µg/L
Attribute:
Attribute Label: PbTx-1 Air
Attribute Definition: brevetoxin concentration in marine aerosols
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U30
Range Domain Maximum: TBD
Attribute Units of Measure: ng/M^3
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: PbTx-1 Sed
Attribute Definition: brevetoxin concentrations in surficial sediments
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U1.7
Range Domain Maximum: TBD
Attribute Units of Measure: µg/g
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: PbTx-2
Attribute Definition: concentration of brevetoxin-2(number) . Detection limits are based on sea water samples.
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: U0.1
Range Domain Maximum: TBA
Attribute Units of Measure: µg/L
Attribute:
Attribute Label: PbTx-2 Air
Attribute Definition: brevetoxin concentration in marine aerosols
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U4
Range Domain Maximum: TBD
Attribute Units of Measure: ng/M^3
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: PbTx-2 Sed
Attribute Definition: brevetoxin concentrations in surficial sediments
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U0.2
Range Domain Maximum: TBD
Attribute Units of Measure: µg/g
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: PbTx-3
Attribute Definition: concentration of brevetoxin-3(number) . Detection limits are based on sea water samples.
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: U0.2
Range Domain Maximum: TBA
Attribute Units of Measure: µg/L
Attribute:
Attribute Label: PbTx-3 Air
Attribute Definition: brevetoxin concentration in marine aerosols
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U9
Range Domain Maximum: TBD
Attribute Units of Measure: ng/M^3
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: PbTx-3 Sed
Attribute Definition: brevetoxin concentrations in surficial sediments
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U0.5
Range Domain Maximum: TBD
Attribute Units of Measure: µg/g
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: PbTx-5
Attribute Definition: concentration of brevetoxin-5(number) . Detection limits are based on sea water samples.
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: U0.2
Range Domain Maximum: TBA
Attribute Units of Measure: µg/L
Attribute:
Attribute Label: PbTx-5 Air
Attribute Definition: brevetoxin concentration in marine aerosols
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U10
Range Domain Maximum: TBD
Attribute Units of Measure: ng/M^3
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: PbTx-5 Sed
Attribute Definition: brevetoxin concentrations in surficial sediments
Attribute Domain Values:
Range Domain:
Range Domain Minimum: U0.6
Range Domain Maximum: TBD
Attribute Units of Measure: µg/g
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Detailed Description:
Entity Type:
Entity Type Label: Hydrography
Entity Type Definition: Physical parameters (CTD) associated with red tide blooms.
Attribute:
Attribute Label: C1S/m
Attribute Definition: Water conductivity as measured by the CTD's secondary conductivity sensor.
Attribute Domain Values:
Range Domain:
Attribute Units of Measure: mmhos
Attribute:
Attribute Label: COS/m
Attribute Definition: Water conductivity as measured by the CTD's primary conductivity sensor.
Attribute Domain Values:
Range Domain:
Attribute Units of Measure: mmhos
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: Sal00
Attribute Definition: Water salinity as measured by the CTD primary sensor (number)
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: 40
Attribute Units of Measure: ppt
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: Sal11
Attribute Definition: Water salinity as measured by the CTD secondary sensor (number)
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: 40
Attribute Units of Measure: ppt
Attribute:
Attribute Label: Sigmat11
Attribute Definition: Water density as measured by the secondary temperature and conductivity sensors.
Attribute:
Attribute Label: Stigma-t00
Attribute Definition: Water density as measured by the primary temperature and conductivity sensors.
Attribute:
Attribute Label: T068
Attribute Definition: Water temperature as measured by the CTD primary sensor (number).
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: 40
Attribute Units of Measure: degrees C
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: T168
Attribute Definition: Water temperature as measured by the CTD secondary sensor.
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: 40
Attribute Units of Measure: degrees C
Detailed Description:
Entity Type:
Entity Type Label: HPLC pigments
Entity Type Definition: Biological pigments associated with phytoplankton in red tide blooms
Attribute:
Attribute Label: 19'-butanoyloxyfuc
Attribute Definition: concentration of pigment
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Attribute:
Attribute Label: 19'-hexanoyloxyfuc
Attribute Definition: concentration of pigment
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Attribute:
Attribute Label: 9'-cis-neoxanthin
Attribute Definition: concentration of pigment
Attribute Domain Values:
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Attribute:
Attribute Label: alloxanthin
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: alpha carotene
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: antheraxanthin
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: beta-carotene
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: chlorophyll a1
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: chlorophyll b1
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: chlorophyll c1&c2
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: chlorophyll c3
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: chlorophyllide a
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: cis-fucoxanthin
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: diadinoxanthin
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: diatoxanthin
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: divinyl chlorophyll a
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: fucoxanthin
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: gyroxanth-diester
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: lutein
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: Nutrient sample
Attribute Definition: X
Attribute:
Attribute Label: peridinin
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: phaeophytin a
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: Tray
Attribute Definition: x
Attribute:
Attribute Label: violoxanthin
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Attribute:
Attribute Label: zeaxanthin
Attribute Definition: concentration of pigment
Attribute Domain Values:
Range Domain:
Range Domain Minimum: 0
Range Domain Maximum: TBD
Attribute Units of Measure: µg/L
Enumerated Domain:
Enumerated Domain Value: -999
Enumerated Domain Value Definition: default value
Overview Description:
Entity and Attribute Overview: Parameters measured to help describe physical, chemical and biological processes associated with red tide blooms
Distribution Information
Section Index
Distributor:
Contact Information:
Contact Person Primary:
Contact Person: Dr. Gary Kirkpatrick
Contact Organization: Mote Marine Laboratory
Contact Position: Senior Scientist
Contact Address:
Address Type: mailing and physical address
Address: 1600 Ken Thompson Parkway
City: Sarasota
State or Province: FL
Postal Code: 34236
Country: US
Contact Voice Telephone: (941) 388-4441
Contact Facsimile Telephone: (941) 388-4312
Contact Electronic Mail Address: gkirkpat@mote.org
Metadata Reference Information
Section Index
Metadata Date: 8/29/2000
Metadata Review Date: 7/2/2001
Metadata Future Review Date:
Metadata Contact:
Contact Information:
Contact Organization Primary:
Contact Organization: Florida Marine Research Institute (FWC)
Contact Person: Teresa Steely
Contact Position: Metadata Specialist
Contact Address:
Address Type: mailing and physical address
Address: 100 8th Ave SE
City: St. Petersburg
State or Province: FL
Postal Code: 33701
Country: US
Contact Voice Telephone: (727) 896-8626 ext. 2035
Contact Facsimile Telephone: (727) 823-0166
Contact Electronic Mail Address: Teresa.Steely@fwc.state.fl.us
Metadata Standard Name: FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata
Metadata Standard Version: February 1999
Metadata Time Convention: local time
Metadata Access Constraints: None. This metadata is public information.
Metadata Use Constraints: None.
Metadata Security Information:
Metadata Security Classification System: None.
Metadata Security Classification: Unclassified
Metadata Security Handling Description: None.
SMMS Metadata report generated 7/2/2001